Chromatographic estimation of p-coumaric acid and triacontanol in an Ayurvedic root drug patala (Stereospermum suaveolens Roxb.).

Abstract

A high-performance thin layer chromatographic method has been established for the identification and standardization of Stereospermum suaveolens extracts using two active biomarkers viz. triacontanol and p-coumaric acid in root samples collected from different geographical locations of India. These marker components have different therapeutic activities: p-coumaric acid is a good antioxidant and also has anti-diabetic properties; triacontanol has anti-inflammatory and anti-ulcer properties. Studies on root as well as stem samples showed characteristic bands of triacontanol and p-coumaric acid at R(f) 0.51 and 0.42, respectively. The amount of triacontanol varied from 0.035-8.472 mg/g in the root samples while the amount of p-coumaric acid was in the range 0.081-0.203 mg/g. The limit of detection for triacontanol was 100 ng/spot whereas that for p-coumaric acid was 10 ng/spot.

• Authors: Neena Srivastava, Sayadda Khatoon, A K S Rawat, Vartika Rai, Shanta Mehrotra
• Journal: Journal of chromatographic science (J Chromatogr Sci) 2009 Nov-Dec Vol. 47 Issue 10 Pg. 936-9 ISSN: 1945-239X [Electronic] United States
• PMID: 19930809 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Coumaric Acids
• Fatty Alcohols
• Plant Extracts
• Propionates
• 1-triacontanol
• p-coumaric acid

Topics

• Bignoniaceae (chemistry)
• Chromatography, High Pressure Liquid (methods)
• Coumaric Acids (analysis)
• Fatty Alcohols (analysis)
• Medicine, Ayurvedic
• Plant Extracts (analysis)
• Plant Roots (chemistry)
• Propionates