Abstract | BACKGROUND: OBJECTIVE: To measure the EGF and amphiregulin concentrations in sputa obtained from patients with asthma under various conditions, and to examine the effects of EGF and amphiregulin on the proliferation or differentiation of airway structural cells. METHODS:
Epidermal growth factor and amphiregulin levels were measured by ELISA in sputum specimens collected from 14 hospitalized children with asthma during an acute asthma attack, 13 stable outpatients with asthma, 8 healthy control children, and 7 children with respiratory tract infections. The effects of EGF and amphiregulin on the proliferation and/or differentiation of normal human bronchial epithelial cells (NHBE), bronchial smooth muscle cells (BSMC), and normal human lung fibroblasts (NHLF) were examined. RESULTS: The sputum levels of EGF were significantly higher for about a week after an acute asthma attack compared with the levels in stable subjects with asthma and control subjects. In contrast, upregulation of amphiregulin in the sputa of patients with asthma was observed only during the acute attack. EGF caused proliferation of NHBE, BSMC, and NHLF, whereas amphiregulin induced proliferation of only NHBE. Prolonged exposure of NHBE to EGF and amphiregulin induced mucous cell metaplasia in an IL-13-independent manner. CONCLUSION:
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Authors | Yukinori Enomoto, Kanami Orihara, Tetsuya Takamasu, Akio Matsuda, Yasuhiro Gon, Hirohisa Saito, Chisei Ra, Yoshimichi Okayama |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 124
Issue 5
Pg. 913-20.e1-7
(Nov 2009)
ISSN: 1097-6825 [Electronic] United States |
PMID | 19895983
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AREG protein, human
- Amphiregulin
- EGF Family of Proteins
- Glycoproteins
- Intercellular Signaling Peptides and Proteins
- MUC5AC protein, human
- Mucin 5AC
- Proto-Oncogene Proteins c-fos
- Recombinant Proteins
- Epidermal Growth Factor
- Extracellular Signal-Regulated MAP Kinases
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Topics |
- Acute Disease
- Adolescent
- Amphiregulin
- Asthma
(metabolism, pathology)
- Cell Line
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Child
- Child, Preschool
- EGF Family of Proteins
- Epidermal Growth Factor
(analysis, metabolism, pharmacology)
- Epithelial Cells
(drug effects, metabolism, pathology)
- Extracellular Signal-Regulated MAP Kinases
(drug effects, metabolism)
- Female
- Fibroblasts
(drug effects, metabolism)
- Glycoproteins
(analysis, metabolism, pharmacology)
- Humans
- Infant
- Infant, Newborn
- Intercellular Signaling Peptides and Proteins
(analysis, metabolism, pharmacology)
- Male
- Mucin 5AC
(agonists, metabolism)
- Myocytes, Smooth Muscle
(drug effects, metabolism)
- Phosphorylation
(drug effects, physiology)
- Proto-Oncogene Proteins c-fos
(agonists, metabolism)
- Recombinant Proteins
(pharmacology)
- Respiratory Mucosa
(drug effects, metabolism, pathology)
- Signal Transduction
(drug effects, physiology)
- Sputum
(chemistry)
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