Abstract | AIM: METHODS: The toxic concentration range of flavonoids was evaluated using the MTT assay. The ability of MDA-MB-231 cells to invade was evaluated using a modified Boyden chamber system. The activity of MMP-3 was determined by casein zymography. The secretion of MMP-3 was evaluated using Western blotting, casein zymography and confirmed by ELISA. RESULTS: Some putative flavonoids, ie, quercetin and kaempferol ( flavonols), significantly inhibited the in vitro invasion of MDA-MB-231 cells in a concentration-dependent manner, with IC(50) values of 27 and 30 micromol/L, respectively. Quercetin and kaempferol also reduced MMP-3 activity in a dose-dependent manner, with IC(50) values in the range of 30 micromol/L and 45 micromol/L, respectively. None of the flavonoids had a significant effect on the secretion of MMP-3. CONCLUSION:
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Authors | Kanokkarn Phromnoi, Supachai Yodkeeree, Songyot Anuchapreeda, Pornngarm Limtrakul |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 30
Issue 8
Pg. 1169-76
(Aug 2009)
ISSN: 1745-7254 [Electronic] United States |
PMID | 19617894
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Flavonoids
- Matrix Metalloproteinase Inhibitors
- Matrix Metalloproteinase 3
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Topics |
- Breast Neoplasms
(pathology)
- Carcinoma
(pathology)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Survival
(drug effects)
- Female
- Flavonoids
(therapeutic use)
- Humans
- Matrix Metalloproteinase 3
(metabolism)
- Matrix Metalloproteinase Inhibitors
- Neoplasm Invasiveness
(prevention & control)
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