Abstract |
IL-4 induces a lipase, pancreatic lipase related protein 2 ( PLRP2), in cytotoxic T lymphocytes (CTLs). Because PLRP2 in semen can mediate lipid-dependent toxicity to sperm, we questioned whether CTL-derived PLRP2 could support similar cytotoxicity toward tumor cells. Recombinant PLRP2 was toxic to P815 tumor cells in 48 h when lipid and another protein, colipase, were present. However, PLRP2-positive CTLs (induced with many lots of IL-4) were unable to mediate lipid-dependent cytotoxicity. Notably, CTLs induced with only one lot of IL-4 had lipid-dependent cytotoxicity. The exceptional lot of IL-4 was effective in multiple experiments at inducing lipid-dependent cytotoxicity. The lipid-dependent cytotoxicity it induced was determined to be perforin-independent. CTLs induced with IL-4 that was unable to induce lipid-dependent cytotoxicity had mRNA for PLRP2 but not mRNA for colipase. Therefore, we added exogenous colipase to the CTL assays but still cytotoxicity was unchanged. We conclude (1) that lipid-dependent cytotoxicity, promoted by the lipase PLRP2 and colipase, will kill tumor cells and (2) that more than PLRP2 alone is required for lipid-dependent cytotoxicity mediated by CTLs.
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Authors | Bryce N Alves, Kristen Marshall, David L Tamang, Jeffrey Leong, Doug Redelman, Viki Elliott, Mark E Lowe, Dorothy Hudig |
Journal | Cell biochemistry and function
(Cell Biochem Funct)
Vol. 27
Issue 5
Pg. 296-308
(Jul 2009)
ISSN: 1099-0844 [Electronic] England |
PMID | 19548271
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | (c) 2009 John Wiley & Sons, Ltd. |
Chemical References |
- Colipases
- Recombinant Proteins
- Triglycerides
- Interleukin-4
- Linoleic Acid
- Lipase
- pancreatic lipase related protein 2
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Topics |
- Animals
- Cell Line, Tumor
- Colipases
(pharmacology, toxicity)
- Cytotoxicity, Immunologic
- Humans
- Interleukin-4
(metabolism)
- Jurkat Cells
- Linoleic Acid
(pharmacology, toxicity)
- Lipase
(pharmacology, toxicity)
- Mice
- Mice, Inbred BALB C
- Recombinant Proteins
(pharmacology, toxicity)
- T-Lymphocytes, Cytotoxic
(enzymology, immunology)
- Triglycerides
(pharmacology, toxicity)
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