Microarray analysis of microRNA expression in renal clear cell carcinoma.

Abstract

AIM:

To explore the microRNA (miRNA) expression in renal clear cell carcinoma (RCCC).

METHODS:

We compared the miRNA expression profiles in 11 pairs of RCCC and adjacent nontumorous tissue (NT) from 11 RCCC patients, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. To verify microarray results, Northern blotting was carried out on 5 randomly selected miRNAs.

RESULTS:

Totally 81 miRNAs were identified valid expression in RCCC samples, 48 of which specifically detected in RCCC samples, 17 of which detected downregulated in RCCC compared to NT sample, 2 upregulated and 14 without significant difference. MiRNAs in RCCC tissues exhibit an overall higher expression level than NT tissue. The chip results were confirmed by northern blot analysis.

CONCLUSION:

Our study may help to clarify the molecular mechanisms involved in the pathogenesis of RCCC, and miRNAs potentially serve as a novel diagnostic biomarker of RCCC.

• Authors: Y Huang, Y Dai, J Yang, T Chen, Y Yin, M Tang, C Hu, L Zhang
• Journal: European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology (Eur J Surg Oncol) Vol. 35 Issue 10 Pg. 1119-23 (Oct 2009) ISSN: 1532-2157 [Electronic] England
• PMID: 19443172 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• MIRN145 microRNA, human
• MIRN17 microRNA, human
• MIRN221 microRNA, human
• MicroRNAs

Topics

• Adult
• Biomarkers, Tumor (genetics, metabolism)
• Carcinoma, Renal Cell (genetics, physiopathology)
• Gene Expression Regulation, Neoplastic
• Humans
• Kidney Neoplasms (genetics, physiopathology)
• Male
• MicroRNAs (metabolism)
• Microarray Analysis
• Middle Aged