Abstract |
Clathrin- and caveolae-mediated endocytosis have been implicated in the productive entry of many viruses into host cells. ADP-ribosylation factor 6 (Arf6)-dependent endocytosis is another endocytosis pathway that traffics from the cell surface and it is the only Arf that traffics at the plasma membrane. However, little is known about Arf6-dependent trafficking during virus entry. This study showed that coxsackievirus type B3 (CVB3) associated with decay-accelerating factor in non-polarized HeLa cells can be redirected into non-productive compartments by Arf6-dependent internalization, thus restricting infection. Overexpression of wild-type (WT) and constitutively active (CA) Arf6 in HeLa cells resulted in a 2.3- and 3.6-fold decrease in infection, respectively. A dominant-negative inhibitor of Arf6 recovered restriction of infection by WT-Arf6 and CA-Arf6. RNA interference of endogenous Arf6 resulted in a 3.3-fold increase in CVB3 titre in HeLa cells. It was shown that coxsackie-adenovirus receptor (CAR) ligation by virus or CAR-specific antibody could activate extracellular signal-regulated kinase (ERK) of the mitogen-activated protein kinase family and lead to Arf6-mediated viral restriction. In the absence of ERK activation, CVB3 internalization into early endosomes was inhibited and subsequent infection was reduced, but Arf6-mediated restriction was also abolished. In conclusion, receptor-mediated signalling enhances CVB3 entry whilst also activating non-productive pathways of virus entry; thus, virus infection is an equilibrium of productive and non-productive pathways of entry.
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Authors | David Marchant, Alhousseynou Sall, Xiaoning Si, Thomas Abraham, Winnie Wu, Zongshu Luo, Tamar Petersen, Richard G Hegele, Bruce M McManus |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 90
Issue Pt 4
Pg. 854-862
(Apr 2009)
ISSN: 0022-1317 [Print] England |
PMID | 19264606
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ADP-Ribosylation Factor 6
- CLMP protein, human
- Coxsackie and Adenovirus Receptor-Like Membrane Protein
- Receptors, Virus
- Extracellular Signal-Regulated MAP Kinases
- ADP-Ribosylation Factors
- ARF6 protein, human
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Topics |
- ADP-Ribosylation Factor 6
- ADP-Ribosylation Factors
(genetics, metabolism)
- Cell Line
- Coxsackie and Adenovirus Receptor-Like Membrane Protein
- Endocytosis
- Endosomes
(virology)
- Enterovirus B, Human
(genetics, metabolism, pathogenicity)
- Extracellular Signal-Regulated MAP Kinases
(genetics, metabolism)
- HeLa Cells
- Humans
- Receptors, Virus
(genetics, metabolism)
- Signal Transduction
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