Effects of intramammary infusion of cis-urocanic acid on mastitis-associated inflammation and tissue injury in dairy cows.

Abstract

OBJECTIVE:

To evaluate the effects of cis-urocanic acid (cis-UCA) on mammary gland (MG) inflammation and injury associated with Escherichia coli-induced mastitis in dairy cows.

ANIMALS:

12 lactating dairy cows (36 MGs).

PROCEDURES:

At 7-week intervals, a different MG in each cow was experimentally inoculated with E coli. At 6-hour intervals from 6 to 36 hours after inoculation, the inoculated MG in each cow was infused with 40 mL of saline (0.9% NaCl) solution, 12.5mM cis-UCA, or 25mM cis-UCA (4 cows/group); ultimately, each cow received each treatment. Immediately prior to and at various time points after inoculation and treatment, milk samples were collected. Bacterial CFUs, somatic cell counts (SCCs), N-acetyl-beta-D-glucosaminidase (NAGase) and lactate dehydrogenase (LDH) activities, and concentrations of bovine serum albumin, tumor necrosis factor-alpha, and cis-UCA were quantified in each milk sample. Results-Compared with findings in saline solution-treated MGs, NAGase and LDH activities in milk samples from cis-UCA-treated MGs were lower. Cis-UCA had no effect on milk SCCs and milk concentrations of bovine serum albumin and tumor necrosis factor-alpha. Furthermore, cis-UCA had no adverse effect on bacterial clearance; CFUs of E coli in MGs treated with saline solution or cis-UCA were equivalent.

CONCLUSIONS AND CLINICAL RELEVANCE:

In cows, milk NAGase and LDH activities were both lower in E coli-infected MGs infused with cis-UCA than in those infused with saline solution, which suggests that cis-UCA reduced mastitis-associated tissue damage. Furthermore, these data indicated that therapeutic concentrations of cis-UCA in milk can be achieved via intramammary infusion.

• Authors: Douglas D Bannerman, Manuela Rinaldi, Bryan T Vinyard, Jarmo Laihia, Lasse Leino
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 70 Issue 3 Pg. 373-82 (Mar 2009) ISSN: 0002-9645 [Print] United States
• PMID: 19254150 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Urocanic Acid

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Cattle
• Dose-Response Relationship, Drug
• Escherichia coli (drug effects)
• Escherichia coli Infections (drug therapy, veterinary)
• Female
• Inflammation (drug therapy)
• Mastitis, Bovine (drug therapy)
• Time Factors
• Urocanic Acid (administration & dosage, pharmacology)