Adenosine receptors in COPD and asymptomatic smokers: effects of smoking cessation.

Abstract

Our group has shown that 1-year smoking cessation persisted or increased airway inflammation in chronic obstructive pulmonary disease (COPD). We compared adenosine and adenosine receptor (AR) expression in COPD and asymptomatic smokers (AS) before and after 1-year smoking cessation. Sputum cytospins and bronchial biopsies of (ex)smoking COPD patients and AS were studied for A(1)R, A(2A)R, A(2B)R, and A(3)R expression. Adenosine and inflammatory mediators were measured in sputum supernatants. At baseline, COPD patients had lower levels of adenosine and higher levels of vascular endothelial growth factor in sputum than AS. Smoking cessation induced significantly different effects in COPD than in AS, i.e. an increase in percentages of A(3)R expressing neutrophils and A(1)R expressing macrophages in COPD as increase in adenosine and monocyte chemoattractant protein-1 levels in sputum. Adenosine-related effector mechanisms may contribute to the persistence and progression of airway inflammation in COPD following 1-year smoking cessation.

• Authors: Mieke Versluis, Nick ten Hacken, Dirkje Postma, Begona Barroso, Bea Rutgers, Marie Geerlings, Brigitte Willemse, Wim Timens, Machteld Hylkema
• Journal: Virchows Archiv : an international journal of pathology (Virchows Arch) Vol. 454 Issue 3 Pg. 273-81 (Mar 2009) ISSN: 1432-2307 [Electronic] Germany
• PMID: 19165499 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Inflammation Mediators
• Receptors, Purinergic P1
• Adenosine

Topics

• Adenosine (metabolism)
• Adult
• Bronchoscopy
• Chromatography, High Pressure Liquid
• Disease Progression
• Female
• Humans
• Inflammation (metabolism, pathology)
• Inflammation Mediators (metabolism)
• Male
• Middle Aged
• Pulmonary Disease, Chronic Obstructive (metabolism, pathology)
• Receptors, Purinergic P1 (metabolism)
• Smoking (adverse effects)
• Smoking Cessation
• Sputum (chemistry, metabolism)