[A comparison study of post-cerebral hemorrhage stress ulcer with two interventional treatments aimed at endogenous endothelin in rat].

Abstract

OBJECTIVE:

To observe the respective effects of intervention either with endothelin (ET) antibody or with ET receptor antagonist on acute stress ulcer (ASU) subsequent to cerebral hemorrhage in rats.

METHODS:

Forty Sprague-Dawley (SD) rats were randomly divided into control group (group A, n=10), model group (group B, n=10), ET antibody (group C, n=10), and ET receptor antagonist group (group D, n=10). Right intracerebral hemorrhage was reproduced by injection of 200 microl autologous venous blood. Normal saline, ET antibody, or ET receptor antagonist was respectively administered intravenously per day for designated group. The rats were sacrificed at 3 days of the experiment. The incidence of ASU and ulcer index were assessed, serum ET-1 level, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in serum, Rsvmit (Rsv) and Vvmit (Vv) of cerebral and gastric mucosa were measured, and pathological examination of the cerebral tissue and gastric mucosa was performed with light microscopy and electron microscopy.

RESULTS:

In group B, serum ET-1 level did not changed. MDA content were markedly increased in serum, cerebral and gastric mucosa, SOD activity were markedly decreased, cerebral water content were markedly increased; Rsv in neuron and gastric parietal cell, Vv in gastric parietal cell both were markedly decreased (P<0.05 or P<0.01). ASU was only observed in group B (the incidence was 30%, ulcer index was 15). It was not observed in other groups. Compared with group B, MDA content were significantly decreased, and SOD activity were significantly increased in serum, cerebral and gastric mucosa in groups C and D, cerebral water content in group C were dramatically decreased (all P<0.01), but these were not statistically different between groups C and D. Rsv and Vv in neuron and gastric parietal cell in groups B, C and D were not statistically different, and serum ET-1 level were not statistically different among the groups (all P>0.05).

CONCLUSION:

Intervention of ET antibody and ET receptor antagonist can both reduce occurrence and development of ASU subsequent to cerebral hemorrhage in rats.

• Authors: Xing-qing Huang, Wen-lin Rao, Xiao-yan Liu, Yan-dong Zhong, Tuo Lin, Chang-rui Zhang, Biao Zhu
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 20 Issue 11 Pg. 678-80 (Nov 2008) ISSN: 1003-0603 [Print] China
• PMID: 19000428 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Ulcer Agents
• Antibodies
• Endothelin Receptor Antagonists
• Endothelin-1
• Endothelins
• Malondialdehyde
• Superoxide Dismutase

Topics

• Animals
• Anti-Ulcer Agents (therapeutic use)
• Antibodies (therapeutic use)
• Brain (metabolism, pathology)
• Cerebral Hemorrhage (complications, metabolism, pathology)
• Disease Models, Animal
• Endothelin Receptor Antagonists
• Endothelin-1 (blood)
• Endothelins (immunology)
• Gastric Mucosa (metabolism)
• Male
• Malondialdehyde (metabolism)
• Peptic Ulcer (etiology, prevention & control)
• Rats
• Rats, Sprague-Dawley
• Stomach (pathology)
• Superoxide Dismutase (metabolism)