Abstract | OBJECTIVE: METHODS: To see whether antibodies from patients with NT- and T-LEMS differentially recognize antigenic sites of the alpha 1A subunit of P/Q-type VGCC, we studied serum samples from 22 T-LEMS and 24 NT-LEMS patients. Sera reactivity was tested by Western blot analysis to recombinant proteins corresponding to the extracellular S5-S6 linker region of three out of four domains forming the alpha 1 subunit of P/Q-type VGCC. RESULTS: Sera from 9/24 (37,5%) NT-LEMS patients, but only 1/22 (4,6%) T-LEMS patients recognized domain IV (p=0,011). Seroreactivity to domains I and III was similar for NT-LEMS and T-LEMS patients (domain I: 8%/14%; domain III: 46%/41%, not significant). CONCLUSIONS: These data suggest that an antibody response to domain IV is more common in LEMS without tumor than in paraneoplastic LEMS. This may have implications for diagnostic workup in LEMS patients without previously established diagnosis of a tumor. Additionally this could point towards a differential autoimmune pathogenesis between T-LEMS and NT-LEMS.
|
Authors | Hannah L Pellkofer, Lena Armbruster, Markus Krumbholz, Maarten J Titulaer, Jan J Verschuuren, Friedrich Schumm, Raymond Voltz |
Journal | Journal of neuroimmunology
(J Neuroimmunol)
Vol. 204
Issue 1-2
Pg. 136-9
(Nov 15 2008)
ISSN: 0165-5728 [Print] Netherlands |
PMID | 18809213
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Autoantibodies
- Calcium Channels, N-Type
- voltage-dependent calcium channel (P-Q type)
|
Topics |
- Autoantibodies
(blood)
- Calcium Channels, N-Type
(immunology)
- Female
- Humans
- Lambert-Eaton Myasthenic Syndrome
(blood, complications)
- Lung Neoplasms
(blood)
- Male
- Middle Aged
- Protein Structure, Tertiary
- Small Cell Lung Carcinoma
(blood)
|