Induction of apoptosis by KI0477959 through activation of caspase-3 in human leukemia cell line, HL-60 cells.

Abstract

KI0477959 (Herbkines) has been used for the purpose of development of physical strength in wasting diseases, like cancer. In the present study, apoptosis-inducing activities of butanol fraction of KI0477959 were studied in human leukemia cell line, HL-60 cells. KI0477959 increased cytotoxicity but had less effect on human peripheral blood mononuclear cells. KI0477959-induced apoptosis was accompanied by activation of caspase-3 and specific proteolytic cleavage of poly-ADP-ribose polymerase. Increased apoptosis was reduced by treatment with p38 and extracellular signal-regulated protein kinase (ERK) inhibitors. These results suggest that KI0477959 induces apoptosis through activation of caspase-3, p38, and ERK in HL-60 cells.

• Authors: Hyun-Ja Jeong, Ju-Young Lee, Joon-Bae Kim, Hoyeon Go, Seong-Gyu Ko, Young-Wan Seo, Sejin Jeong, Jinhan Park, Ho-Jeong Na, Jae-Young Um, Hyung-Min Kim, Seung-Heon Hong
• Journal: The International journal of neuroscience (Int J Neurosci) Vol. 118 Issue 10 Pg. 1384-99 (Oct 2008) ISSN: 1563-5279 [Electronic] England
• PMID: 18788024 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• COL11A2 protein, human
• Collagen Type XI
• Drug Combinations
• Drugs, Chinese Herbal
• Herbkines
• Plant Extracts
• Mitogen-Activated Protein Kinase Kinases
• Caspase 3

Topics

• Analysis of Variance
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Cycle (drug effects)
• Cell Survival (drug effects)
• Collagen Type XI (metabolism)
• DNA Fragmentation (drug effects)
• Drug Combinations
• Drugs, Chinese Herbal (chemistry, pharmacology)
• HL-60 Cells
• Humans
• Leukemia (metabolism, pathology)
• Medicine, Korean Traditional
• Mitogen-Activated Protein Kinase Kinases (metabolism)
• Plant Extracts (chemistry, pharmacology)
• Time Factors