Intracellular and extracellular expression of the major inducible 70kDa heat shock protein in experimental ischemia-reperfusion injury of the spinal cord.

Abstract

Inflammatory responses exacerbate ischemia-reperfusion (IR) injury of spinal cord, although understanding of mediators is incomplete. The major inducible 70kDa heat shock protein (hsp70) is induced by ischemia and extracellular hsp70 (e-hsp70) can modulate inflammatory responses, but there is no published information regarding e-hsp70 levels in the cerebrospinal fluid (CSF) or serum as part of any neurological disease state save trauma. The present work addresses this deficiency by examining e-hsp70 in serum and CSF of dogs in an experimental model of spinal cord IR injury. IR injury of spinal cord caused hind limb paraplegia within 2-3 h that was correlated to lumbosacral poliomalacia with T cell infiltrates at 3 d post-ischemia. In this context, we showed a 5.2-fold elevation of e-hsp70 in CSF that was induced by ischemia and was sustained for the following 3 d observation interval. Plasma e-hsp70 levels were unaffected by IR injury, indicating e-hsp70 release from within the central nervous system. A putative source of this e-hsp70 was ependymal cells in the ischemic penumbra, based upon elevated i-hsp70 levels detected within these cells. Results warrant further investigation of e-hsp70's potential to modulate spinal cord IR injury.

• Authors: Hamdy Awad, Zacharias Suntres, John Heijmans, Daniel Smeak, Valerie Bergdall-Costell, Fievos L Christofi, Cynthia Magro, Michael Oglesbee
• Journal: Experimental neurology (Exp Neurol) Vol. 212 Issue 2 Pg. 275-84 (Aug 2008) ISSN: 1090-2430 [Electronic] United States
• PMID: 18511046 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HSP70 Heat-Shock Proteins
• Interleukin-3
• Lipid Peroxides
• Recombinant Fusion Proteins
• Recombinant Proteins
• myelopoietin
• Granulocyte Colony-Stimulating Factor
• Thromboxane B2

Topics

• Animals
• Disease Models, Animal
• Dogs
• Gene Expression (physiology)
• Gene Expression Regulation (physiology)
• Granulocyte Colony-Stimulating Factor (metabolism)
• HSP70 Heat-Shock Proteins (blood, cerebrospinal fluid)
• Interleukin-3 (metabolism)
• Lipid Peroxides (cerebrospinal fluid)
• Motor Neurons (metabolism)
• Recombinant Fusion Proteins (metabolism)
• Recombinant Proteins
• Reperfusion Injury (metabolism, pathology)
• Spinal Cord (pathology)
• Spinal Cord Ischemia (metabolism, pathology)
• Thromboxane B2 (metabolism)
• Time Factors