Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling.

Abstract

AIM:

To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells.

METHODS:

The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI).

RESULTS:

By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non-apoptotic cells decreased.

CONCLUSION:

NGVEV can induce the infected DEF cells to undergo apoptosis and the apoptosis occurs prior to necrosis.

• Authors: Shun Chen, An-Chun Cheng, Ming-Shu Wang, Xi Peng
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 14 Issue 14 Pg. 2174-8 (Apr 14 2008) ISSN: 1007-9327 [Print] United States
• PMID: 18407590 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Annexin A5
• Propidium
• Fluorescein-5-isothiocyanate
• Fluorescein

Topics

• Animals
• Annexin A5 (pharmacology)
• Apoptosis
• Cell Separation
• Fibroblasts (metabolism)
• Flow Cytometry
• Fluorescein (pharmacology)
• Fluorescein-5-isothiocyanate (pharmacology)
• Necrosis
• Propidium (pharmacology)
• Time Factors
• Viruses (metabolism)