Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion rats.

Abstract

Our objective was to evaluate the protective effects of glucagon-like peptide 2 (GLP-2) on intestinal ischemia/reperfusion (I/R) rats. Thirty-two rats were randomly assigned to four experimental groups, each of 8: Group A, sham rats underwent laparotomy only, without superior mesenteric artery (SMA) occlusion; Group B, I/R animals underwent laparotomy and occlusion of the SMA for 60 minutes followed by 120 minutes of reperfusion; Group C, I/R animals underwent intestinal I/R, and received pretreatment with GLP-2 for 3 days preoperatively; and Group D, I/R animals underwent intestinal I/R, received pretreatment with GLP-2 as above, and during the reperfusion phase were injected intravenously with GLP-2. After the reperfusion of intestinal ischemia, samples of intestinal mucosa, mesenteric lymph nodes (MLN) and blood were prepared for determination. In the pretreatment rats with GLP-2 (group C), Chiu's scores, bacterial colony counts, serum D-lactate, intestinal mucosal MDA and ET-1, and serum endotoxin, TNF-alpha and IL-6 were significantly reduced compared with intestinal I/R rats (group B). Administration of GLP-2 during the reperfusion phase following pretreatment (group D) showed further protective effects in comparison with the pretreatment rats (group C). We conclude that treatment with GLP-2 attenuates intestinal I/R injury, reduces bacterial translocation, inhibits the release of oxygen free radicals and ET-1, and may well inhibit the production of proinflammatory cytokines.

• Authors: Wei Zhang, Weiming Zhu, Jian Zhang, Ning Li, Jieshou Li
• Journal: Microsurgery (Microsurgery) Vol. 28 Issue 4 Pg. 285-90 ( 2008) ISSN: 1098-2752 [Electronic] United States
• PMID: 18383347 (Publication Type: Comparative Study, Journal Article)
• Copyright: (c) 2008 Wiley-Liss, Inc. Microsurgery, 2008

Chemical References

• Endothelin-1
• Endotoxins
• Glucagon-Like Peptide 2
• Interleukin-6
• Tumor Necrosis Factor-alpha
• Malondialdehyde

Topics

• Animals
• Disease Models, Animal
• Drug Administration Schedule
• Endothelin-1 (metabolism)
• Endotoxins (blood)
• Glucagon-Like Peptide 2 (administration & dosage, pharmacology)
• Interleukin-6 (blood)
• Intestinal Mucosa (blood supply, drug effects, physiopathology)
• Ischemic Preconditioning (methods)
• Male
• Malondialdehyde (metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (physiopathology, prevention & control)
• Tumor Necrosis Factor-alpha (blood)