Abstract |
The chemoprotective effect of hydroxytyrosol (HT) against Sudan I-induced genotoxicity was investigated in a human hepatoma cell line, HepG2. The comet assay and micronucleus (MN) assay were used to monitor genotoxicity. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe, 2,7-dichlorofluorescein diacetate ( DCFH-DA). The levels of oxidative DNA damage and lipid peroxidation were estimated by immunocytochemistry analysis of 8-hydroxydeoxyguanosine (8-OHdG) and by measuring levels of thiobarbituric acid-reactive substances ( TBARS), respectively. Intracellular glutathione (GSH) level was estimated by fluorometric methods. The results showed that HT significantly reduced the genotoxicity caused by Sudan I. Furthermore, HT ameliorated lipid pexidation as demonstrated by a reduction in TBARS formation and attenuated GSH depletion in a concentration-dependent manner. It was also found that HT reduced intracellular ROS formation and 8-OHdG level caused by Sudan I. These results strongly suggest that HT has significant protective ability against Sudan I-induced genotoxicity.
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Authors | Xiaomei Zhang, Liping Jiang, Chengyan Geng, Cunli Hu, Hiroyuki Yoshimura, Laifu Zhong |
Journal | Free radical research
(Free Radic Res)
Vol. 42
Issue 2
Pg. 189-95
(Feb 2008)
ISSN: 1071-5762 [Print] England |
PMID | 18297612
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Mutagens
- Naphthols
- Reactive Oxygen Species
- 3,4-dihydroxyphenylethanol
- 1-phenylazo-2-naphthol
- 8-Hydroxy-2'-Deoxyguanosine
- Deoxyguanosine
- Glutathione
- Phenylethyl Alcohol
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Topics |
- 8-Hydroxy-2'-Deoxyguanosine
- Antioxidants
(pharmacology)
- Cell Line, Tumor
- DNA Damage
(drug effects)
- Deoxyguanosine
(analogs & derivatives, metabolism)
- Glutathione
(metabolism)
- Humans
- Lipid Peroxidation
(drug effects)
- Micronucleus Tests
- Mutagens
(toxicity)
- Naphthols
(antagonists & inhibitors, toxicity)
- Phenylethyl Alcohol
(analogs & derivatives, pharmacology)
- Reactive Oxygen Species
(metabolism)
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