Gingival crevicular fluid and serum matrix metalloproteinase-8 and tissue inhibitor of matrix metalloproteinase-1 levels in renal transplant patients undergoing different immunosuppressive therapy.

Abstract

AIM:

We investigated gingival crevicular fluid (GCF) and serum matrix metalloproteinase-8 (MMP-8) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) levels from renal transplant patients receiving cyclosporine-A (CsA) and having gingival overgrowth (GO), from patients receiving CsA therapy and having no GO and patients receiving tacrolimus therapy.

MATERIAL AND METHODS:

GCF samples were collected from sites with GO (GO+) and without GO (GO-) in CsA patients having GO; and GO- sites in CsA patients having no GO; sites from tacrolimus, gingivitis and healthy subjects. GCF and serum MMP-8 and TIMP-1 levels were determined by a time-resolved immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay.

RESULTS:

GO+ sites in CsA patients having GO had elevated GCF MMP-8 levels compared with those of CsA patients having no GO, tacrolimus and healthy subjects (p<0.005), but these levels were similar to those of gingivitis. The GCF MMP-8 level was higher in GO+ compared with GO- sites in CsA patients having GO (p<0.05). GCF TIMP-1 levels were similar between groups. Tacrolimus patients had lower GCF MMP-8 levels than gingivitis (p<0.005), but levels similar to the healthy group.

CONCLUSION:

These results show that CsA and tacrolimus therapy has no significant effect on GCF MMP-8 levels, and gingival inflammation seems to be the main reason for their elevations.

• Authors: Gülnur Emingil, Beral Afacan, Taina Tervahartiala, Hüseyin Töz, Gül Atilla, Timo Sorsa
• Journal: Journal of clinical periodontology (J Clin Periodontol) Vol. 35 Issue 3 Pg. 221-9 (Mar 2008) ISSN: 1600-051X [Electronic] United States
• PMID: 18269661 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Immunosuppressive Agents
• Tissue Inhibitor of Metalloproteinase-1
• Cyclosporine
• Matrix Metalloproteinase 8
• Tacrolimus

Topics

• Adolescent
• Adult
• Cyclosporine (adverse effects)
• Epidemiologic Methods
• Female
• Gingival Crevicular Fluid (chemistry)
• Gingival Overgrowth (chemically induced, diagnostic imaging)
• Gingivitis (diagnostic imaging)
• Humans
• Immunocompromised Host
• Immunosuppressive Agents (adverse effects)
• Kidney Transplantation (adverse effects)
• Male
• Matrix Metalloproteinase 8 (blood)
• Middle Aged
• Radiography
• Tacrolimus (adverse effects)
• Tissue Inhibitor of Metalloproteinase-1 (blood)