Abstract | BACKGROUND: AIM: METHODS: Serum samples were taken for routine biochemical investigation from 55 patients with gastric cancer, before treatment, and from 55 control subjects. Total ADH activity was measured photometrically and ALDH activity by a fluorimetric method. For measurement of the activity of class I isoenzymes we used a fluorimetric method, with class-specific fluorogenic substrates. The activity of class III and IV alcohol dehydrogenase was measured photometrically. RESULTS: The activity of the class IV ADH isoenzyme was significantly higher in the sera of patients with gastric cancer. The median activity of this isoenzyme in the total cancer group was approximately 47% higher (7.45 mU/l) than the control level (5.08 mU/l). For this reason total ADH activity was also significantly increased. The activities of other tested ADH isoenzymes and ALDH were unchanged. CONCLUSION: Changes in the activity of, especially, class IV ADH in the sera of patients with gastric cancer seems to be caused by release of the isoenzyme from cancer cells.
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Authors | Wojciech Jelski, Lech Chrostek, Bogdan Zalewski, Maciej Szmitkowski |
Journal | Digestive diseases and sciences
(Dig Dis Sci)
Vol. 53
Issue 8
Pg. 2101-5
(Aug 2008)
ISSN: 0163-2116 [Print] United States |
PMID | 18231859
(Publication Type: Journal Article)
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Chemical References |
- Isoenzymes
- ADH7 protein, human
- Alcohol Dehydrogenase
- formaldehyde dehydrogenase (glutathione)
- Aldehyde Oxidoreductases
- Aldehyde Dehydrogenase
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Topics |
- Aged
- Alcohol Dehydrogenase
(blood)
- Aldehyde Dehydrogenase
(blood)
- Aldehyde Oxidoreductases
(blood)
- Case-Control Studies
- Female
- Humans
- Isoenzymes
- Male
- Middle Aged
- Stomach Neoplasms
(enzymology)
- Up-Regulation
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