Abstract | BACKGROUND: METHODS: We screened for suitable surfactants that exhibited favorable selectivity toward the VLDL remnant (VLDLR) fraction, including intermediate-density lipoproteins (IDLs). We investigated the principal characteristics of this assay by gel filtration of lipoproteins and their particle size distribution. We developed a simple assay and evaluated its performance with the Hitachi-7170 analyzer. RESULTS:
Polyoxyethylene-polyoxybutylene block copolymer (POE-POB) exhibited favorable selectivity toward VLDLR and IDL fractions. POE-POB removed apolipoprotein ( apo) E and apo C-III from IDL particles in the presence of cholesterol esterase (CHER), and the particle size distribution of IDLs became smaller after the reaction. These results revealed that IDL particles are specifically modified in the presence of CHER and POE-POB, making their component cholesterol available for enzymatic assay. Addition of phospholipase D improved the reactivity toward chylomicron remnants (CMRs). We found a high correlation [y = 1.018x- 0.01 mmol/L, r = 0.962 (n = 160)] between the proposed assay and the immunoseparation assay in serum from healthy individuals. CONCLUSION: The homogeneous assay described in this report can measure TRL remnant cholesterol, including CMRs, VLDLRs, and IDLs, with high sensitivity and specificity.
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Authors | Kazuhito Miyauchi, Norihiko Kayahara, Masato Ishigami, Hideyuki Kuwata, Hideharu Mori, Hiroyuki Sugiuchi, Tetsumi Irie, Akira Tanaka, Shizuya Yamashita, Taku Yamamura |
Journal | Clinical chemistry
(Clin Chem)
Vol. 53
Issue 12
Pg. 2128-35
(Dec 2007)
ISSN: 0009-9147 [Print] England |
PMID | 17901111
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cholesterol, VLDL
- Chylomicron Remnants
- Epoxy Compounds
- Indicators and Reagents
- Lipoproteins
- Lipoproteins, IDL
- Surface-Active Agents
- Triglycerides
- remnant-like particle cholesterol
- poly(ethylene oxide)-poly(butylene oxide) copolymer
- Polyethylene Glycols
- Cholesterol
- Phospholipase D
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Topics |
- Autoanalysis
- Cholesterol
(blood)
- Cholesterol, VLDL
(blood)
- Chromatography, Gel
- Chylomicron Remnants
(blood)
- Epoxy Compounds
- Humans
- Hyperlipoproteinemia Type III
(metabolism)
- Indicators and Reagents
- Lipoproteins
(blood)
- Lipoproteins, IDL
(blood)
- Phospholipase D
- Polyethylene Glycols
- Surface-Active Agents
- Triglycerides
(blood)
- Ultracentrifugation
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