[Aqueous humor protein concentration in patients with primary open-angle glaucoma under clinical treatment].

Abstract

PURPOSE:

To compare total protein concentration in the aqueous humor of primary open-angle glaucoma and non-glaucomatous patients.

METHODS:

Aqueous humor samples were obtained from 22 patients just before trabeculectomy for clinically uncontrolled primary open angle glaucoma (POAG group). Aqueous humor (0.1 mL) was aspirated by inserting a 26-gauge needle into the anterior chamber. The same procedure was performed in 22 non-glaucomatous patients just before cataract surgery (control group). Immediately after collection, the aqueous humor was stored at -20 degrees C. Aqueous humor total protein concentration was determined using a colorimetric assay.

RESULTS:

The geometric mean of total protein concentration of the aqueous humor samples was 32 mg/dL (range: 8-137 mg/dL) in the primary open angle glaucoma group and 16 mg/dL (range: 2-85 mg/dL) in the control group. The ratio of the protein concentration between the two groups was 2.0 (95% confidence interval: 1.3 to 3.2; p=0.003).

CONCLUSIONS:

The total protein concentration in primary open-angle glaucoma aqueous humor was approximately two times higher than that in non-glaucomatous subjects.

• Authors: Tiago Dos Santos Prata, Eduardo Vitor Navajas, Luiz Alberto Soares Melo Jr, João Roberto Maciel Martins, Helena Bonciani Nader, Rubens Belfort Jr
• Journal: Arquivos brasileiros de oftalmologia (Arq Bras Oftalmol) 2007 Mar-Apr Vol. 70 Issue 2 Pg. 217-20 ISSN: 0004-2749 [Print] Brazil
• Vernacular Title: Concentração de proteínas no humor aquoso de pacientes com glaucoma primário de ângulo aberto em tratamento clínico.
• PMID: 17589689 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antihypertensive Agents
• Eye Proteins
• Timolol

Topics

• Adult
• Aged
• Antihypertensive Agents (therapeutic use)
• Aqueous Humor (chemistry, drug effects)
• Case-Control Studies
• Cataract (therapy)
• Colorimetry
• Enzyme-Linked Immunosorbent Assay
• Eye Proteins (analysis)
• Female
• Glaucoma, Open-Angle (drug therapy, metabolism, surgery)
• Humans
• Intraocular Pressure
• Male
• Middle Aged
• Preoperative Care
• Prospective Studies
• Timolol (administration & dosage, therapeutic use)
• Trabeculectomy