Abstract | OBJECTIVES: METHODS: The effects of As(2)O(3) on human cervical cancer cell lines (HeLa, SiHa, Caski) adhesion, migration and invasion were observed by means of cell adhesion test, cell migration test and cell invasion test. The effects of As(2)O(3) on p-IkappaB, MMP-2, E-cadherin, caveolin-1 and beta-catenin protein expressions of tumor cells were determined by Western blot. In addition, the effects of As(2)O(3) on NF-kappaB activity of tumor cells were analyzed by immunoblot in whole lysates, cytosol and nucleus, respectively. In animal experiments, cervical cancer cells TC-1 were injected into tail veins of C57BL/6 mice and then the mice were treated by intraperitoneal injection of different doses As(2)O(3). Lung weights and the foci on the surface of lungs were measured. RESULTS:
As(2)O(3) inhibited attachment of tumor cells to Fibronectin and Matrigel, reduced cell motility and inhibited tumor invasion potential. As(2)O(3) treatment also resulted in a positive regulation of caveolin-1, upregulation of E-cadherin and decreased activity of beta-catenin, NF-kappaB and NF-kappaB-regulated gene MMP-2. In animal experiments, lung weights in PBS group (0.31+/-0.07 g) were significantly elevated compared with those in As(2)O(3)-treated groups (0.21+/-0.03 g and 0.17+/-0.03 g) also As(2)O(3) reduced number of metastatic lesions of lungs (15.4+/-3.5 vs. 8.3+/-2.0 and 6.3+/-2.3) in a dose-dependent manner. CONCLUSIONS:
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Authors | Jing Yu, Haili Qian, Yunfeng Li, Yang Wang, Xueyan Zhang, Xiao Liang, Ming Fu, Chen Lin |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 106
Issue 2
Pg. 400-6
(Aug 2007)
ISSN: 0090-8258 [Print] United States |
PMID | 17512576
(Publication Type: Journal Article)
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Chemical References |
- Arsenicals
- Cadherins
- Caveolin 1
- Growth Inhibitors
- NF-kappa B
- Oxides
- beta Catenin
- Arsenic Trioxide
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Topics |
- Animals
- Arsenic Trioxide
- Arsenicals
(pharmacology)
- Cadherins
(biosynthesis)
- Caveolin 1
(biosynthesis)
- Cell Adhesion
(drug effects)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Female
- Growth Inhibitors
(pharmacology)
- HeLa Cells
- Humans
- Lung Neoplasms
(drug therapy, secondary)
- Mice
- Mice, Inbred C57BL
- NF-kappa B
(metabolism)
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Oxides
(pharmacology)
- Uterine Cervical Neoplasms
(drug therapy, metabolism, pathology)
- beta Catenin
(biosynthesis)
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