Abstract |
NF-kappaB transcription factors activate genes important for immune response, inflammation, and cell survival. P-TEFb and DSIF, which are positive and negative transcription elongation factors, respectively, both regulate NF-kappaB-induced transcription, but the mechanism underlying their recruitment to NF-kappaB target genes is unknown. We show here that upon induction of NF-kappaB, a subset of target genes is regulated differentially by either P-TEFb or DSIF. The regulation of these genes and their occupancy by these elongation factors are dependent on the NF-kappaB enhancer and the core promoter type. Converting a TATA-less promoter to a TATA promoter switches the regulation of NF-kappaB from DSIF to P-TEFb. Accumulation or displacement of DSIF and P-TEFb is dictated by the formation of distinct initiation complexes ( TFIID dependent or independent) on the two types of core promoter. The underlying mechanism for the dissociation of DSIF from TATA promoters upon NF-kappaB activation involves the phosphorylation of RNA polymerase II by P-TEFb. The results highlight a regulatory link between the initiation and the elongation phases of the transcription reaction and broaden our comprehension of the NF-kappaB pathway.
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Authors | Liat Amir-Zilberstein, Elena Ainbinder, Leanne Toube, Yuki Yamaguchi, Hiroshi Handa, Rivka Dikstein |
Journal | Molecular and cellular biology
(Mol Cell Biol)
Vol. 27
Issue 14
Pg. 5246-59
(Jul 2007)
ISSN: 0270-7306 [Print] United States |
PMID | 17502349
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- NF-kappa B
- Nuclear Proteins
- SUPT5H protein, human
- Transcription Factors
- Transcriptional Elongation Factors
- Phosphoserine
- Positive Transcriptional Elongation Factor B
- RNA Polymerase II
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Topics |
- Animals
- Cell Line
- Chromatin Immunoprecipitation
- Gene Expression Regulation
- Humans
- Jurkat Cells
- Mice
- NF-kappa B
(genetics, metabolism)
- Nuclear Proteins
(metabolism)
- Phosphoserine
(metabolism)
- Positive Transcriptional Elongation Factor B
(metabolism)
- Promoter Regions, Genetic
(genetics)
- RNA Polymerase II
(metabolism)
- TATA Box
(genetics)
- Transcription Factors
(metabolism)
- Transcription, Genetic
- Transcriptional Elongation Factors
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