Abstract |
Three serological tests, enzyme linked immunosorbent assay (ELISA), 50% plaque neutralisation test (50%PNT) and Western blotting (WB), were used to detect antibodies against viral haemorrhagic septicaemia virus (VHSV) in 50 rainbow trout broodstock from a rainbow trout farm endemically infected with VHS but with no clinical signs of infection. When the sera were examined by 50%PNT using the VHSV reference isolate DK-F1 or the heat attenuated DK-F25 mutant strain, no neutralizing antibodies were found. In contrast, when one of the virus isolates from the farm (homologous virus) was used in the 50%PNT, 90% of the fish were found to be positive. By examining a panel of different VHSV isolates in 50%PNT, it was demonstrated that the virus isolate used as test antigen could significantly affect the sensitivity and titre determination in 50%PNT for detection of rainbow trout antibodies against VHSV. When the sera were examined for the presence of VHSV antibodies by ELISA or WB, 61% were found to be positive. When conducting WB analysis, the viral glycoprotein was the protein most frequently recognized, followed by the viral nucleoprotein.
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Authors | J M Fregeneda-Grandes, N J Olesen |
Journal | Diseases of aquatic organisms
(Dis Aquat Organ)
Vol. 74
Issue 2
Pg. 151-8
(Feb 28 2007)
ISSN: 0177-5103 [Print] Germany |
PMID | 17432044
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Antibodies, Viral
(blood)
- Blotting, Western
(veterinary)
- Cell Line
- Enzyme-Linked Immunosorbent Assay
(veterinary)
- Hemorrhagic Septicemia, Viral
(immunology, virology)
- Neutralization Tests
(veterinary)
- Novirhabdovirus
(immunology, isolation & purification)
- Oncorhynchus mykiss
- Sensitivity and Specificity
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