A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate.
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| Abstract |
A target cell-specific activation strategy for improved molecular imaging of peritoneal implants has been proposed, in which fluorophores are activated only in living targeted cells. A current example of an activatable fluorophore is one that is normally self-quenched by attachment to a peptide backbone but which can be activated by specific proteases that degrade the peptide resulting in "dequenching." In this study, an alternate fluorescence activation strategy is proposed whereby self-quenching avidin-rhodamine X, which has affinity for lectin on cancer cells, is activated after endocytosis and degradation within the lysosome. Using this approach in a mouse model of peritoneal ovarian metastases, we document target-specific molecular imaging of submillimeter cancer nodules with minimal contamination by background signal. Cellular internalization of receptor-ligand pairs with subsequent activation of fluorescence via dequenching provides a generalizable and highly sensitive method of detecting cancer microfoci in vivo and has practical implications for assisting surgical and endoscopic procedures.
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| Authors | Yukihiro Hama, Yasuteru Urano, Yoshinori Koyama, Mako Kamiya, Marcelino Bernardo, Ronald S Paik, In Soo Shin, Chang H Paik, Peter L Choyke, Hisataka Kobayashi |
| Journal | Cancer research (Cancer Res) Vol. 67 Issue 6 Pg. 2791-9 (Mar 15 2007) ISSN: 0008-5472 [Print] United States |
| PMID | 17363601 (Publication Type: Journal Article, Research Support, N.I.H., Intramural) |
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