Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium.

Abstract

Using photogenerated glycan arrays, we characterized a large panel of synthetic carbohydrates for their antigenic reactivities with pathogen-specific antibodies. We discovered that rabbit IgG antibodies elicited by Bacillus anthracis spores specifically recognize a tetrasaccharide chain that decorates the outermost surfaces of the B. anthracis exosporium. Since this sugar moiety is highly specific for the spores of B. anthracis, it appears to be a key biomarker for detection of B. anthracis spores and development of novel vaccines that target anthrax spores.

• Authors: Denong Wang, Gregory T Carroll, Nicholas J Turro, Jeffrey T Koberstein, Pavol Kovác, Rina Saksena, Roberto Adamo, Leonore A Herzenberg, Leonard A Herzenberg, Lawrence Steinman
• Journal: Proteomics (Proteomics) Vol. 7 Issue 2 Pg. 180-4 (Jan 2007) ISSN: 1615-9853 [Print] Germany
• PMID: 17205603 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antigens, Bacterial
• Polysaccharides

Topics

• Animals
• Antigens, Bacterial (chemistry, immunology)
• Bacillus anthracis (chemistry, immunology)
• Light
• Microarray Analysis
• Polysaccharides (chemistry, immunology)
• Rabbits
• Spores, Bacterial (chemistry, immunology)