Detection of active human herpesvirus-6 infection in the brain: correlation with polymerase chain reaction detection in cerebrospinal fluid.

Abstract	One-half of bone-marrow transplant (BMT) and stem-cell transplant recipients have reactivation of latent human herpesvirus (HHV)-6 2-4 weeks after transplant. Although the detection of viral DNA, RNA, and antigen in brain material confirmed active HHV-6 variant B infection, peak viral loads in cerebrospinal fluid (CSF) and serum occurred 2-4 weeks before death and decreased to low levels before or at autopsy. All autopsy samples consistently demonstrated HHV-6 active infection in the hippocampus. Astrocytic cells positive for viral antigen provided support for an HHV-6-specific tropism for hippocampal astrocytes. HHV-6 DNA in CSF and serum may not reflect the level of active viral infection in the brain after BMT.
Authors	Julie Fotheringham, Nahid Akhyani, Alexander Vortmeyer, Donatella Donati, Elizabeth Williams, Unsong Oh, Michael Bishop, John Barrett, Juan Gea-Banacloche, Steven Jacobson
Journal	The Journal of infectious diseases (J Infect Dis) Vol. 195 Issue 3 Pg. 450-4 (Feb 01 2007) ISSN: 0022-1899 [Print] United States
PMID	17205485 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References	Antigens, Viral DNA, Viral DNA-Binding Proteins RNA, Messenger RNA, Viral Viral Proteins p41 protein, Human herpesvirus 6
Topics	Antigens, Viral (isolation & purification) Astrocytes (virology) Bone Marrow Transplantation Brain Diseases (diagnosis, pathology, virology) DNA, Viral (blood, cerebrospinal fluid) DNA-Binding Proteins (genetics, isolation & purification) Death Herpesvirus 6, Human (genetics, immunology, isolation & purification) Hippocampus (cytology, virology) Humans Immunohistochemistry Leukocytes, Mononuclear Polymerase Chain Reaction Postoperative Complications RNA, Messenger (analysis) RNA, Viral (analysis) Roseolovirus Infections (diagnosis, pathology, virology) Viral Proteins (genetics, isolation & purification)

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