ADAM10 is a principal 'sheddase' of the low-affinity immunoglobulin E receptor CD23.

Abstract

CD23, the low-affinity immunoglobulin E receptor, is an important modulator of the allergic response and of diseases such as rheumatoid arthritis. The proteolytic release of CD23 from cells is considered a key event in the allergic response. Here we used loss-of-function and gain-of-function experiments with cells lacking or overexpressing candidate CD23-releasing enzymes (ADAM8, ADAM9, ADAM10, ADAM12, ADAM15, ADAM17, ADAM19 and ADAM33), ADAM-knockout mice and a selective inhibitor to identify ADAM10 as the main CD23-releasing enzyme in vivo. Our findings provide a likely target for the treatment of allergic reactions and set the stage for further studies of the involvement of ADAM10 in CD23-dependent pathologies.

• Authors: Gisela Weskamp, Jill W Ford, Jamie Sturgill, Steve Martin, Andrew J P Docherty, Steven Swendeman, Neil Broadway, Dieter Hartmann, Paul Saftig, Shelby Umland, Atsuko Sehara-Fujisawa, Roy A Black, Andreas Ludwig, J David Becherer, Daniel H Conrad, Carl P Blobel
• Journal: Nature immunology (Nat Immunol) Vol. 7 Issue 12 Pg. 1293-8 (Dec 2006) ISSN: 1529-2908 [Print] United States
• PMID: 17072319 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Membrane Proteins
• Receptors, IgE
• Amyloid Precursor Protein Secretases
• ADAM Proteins
• ADAM10 Protein
• Adam10 protein, mouse

Topics

• ADAM Proteins (immunology, metabolism)
• ADAM10 Protein
• Amyloid Precursor Protein Secretases (immunology, metabolism)
• Animals
• B-Lymphocytes (immunology, metabolism)
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• Fibroblasts (immunology, metabolism)
• Flow Cytometry
• Humans
• Immunoblotting
• Membrane Proteins (immunology, metabolism)
• Mice
• Mice, Knockout
• Receptors, IgE (immunology, metabolism)
• Transfection