Role of gp91phox-containing NADPH oxidase in the deoxycorticosterone acetate-salt-induced hypertension.

Abstract

NADPH oxidase plays an important role in vascular oxidative stress in hypertensive diseases. We evaluated whether NADPH oxidase-dependent superoxide (O(2)(-)) production is involved in the deoxycorticosterone acetate (DOCA)-salt-induced hypertension, using mice which are genetically deficient in gp91phox, an NADPH oxidase subunit protein (gp91(-/-) mice). Two weeks after the DOCA-salt treatment, systolic blood pressure was significantly elevated in wild-type mice, but not in gp91(-/-) mice. After a 5-week treatment period, wild-type mice developed high blood pressure, with a systolic blood pressure of 127 +/- 3 mm Hg, compared with 107 +/- 4 mm Hg in gp91(-/-) mice. Aortic O(2)(-) production in wild-type DOCA-salt-treated mice was significantly higher than that in wild-type sham mice, whereas there were no significant differences in aortic O(2)(-) production between gp91(-/-) DOCA-salt-treated and sham mice. These findings suggest that vascular O(2)(-) overproduction via gp91phox-containing NADPH oxidase is one of the crucial factors in the development of DOCA-salt-induced hypertension.

• Authors: Aya Fujii, Daisuke Nakano, Miyuki Katsuragi, Mamoru Ohkita, Masanori Takaoka, Yukihiro Ohno, Yasuo Matsumura
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 552 Issue 1-3 Pg. 131-4 (Dec 15 2006) ISSN: 0014-2999 [Print] Netherlands
• PMID: 17064681 (Publication Type: Journal Article)

Chemical References

• Membrane Glycoproteins
• Superoxides
• Desoxycorticosterone
• Cybb protein, mouse
• NADPH Oxidase 2
• NADPH Oxidases

Topics

• Animals
• Aorta (drug effects, metabolism)
• Blood Pressure (drug effects)
• Desoxycorticosterone (administration & dosage, toxicity)
• Genotype
• Hypertension (chemically induced, genetics, physiopathology)
• Male
• Membrane Glycoproteins (deficiency, genetics, physiology)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• NADPH Oxidase 2
• NADPH Oxidases (deficiency, genetics, physiology)
• Superoxides (metabolism)
• Time Factors