Abstract | AIMS/HYPOTHESIS: SUBJECTS AND METHODS: We studied the effect of four common polymorphisms (rs1920792, I27L, A98V and S487N) in and upstream of the HNF-1alpha gene on transcriptional activity in vitro, and their possible association with type 2 diabetes and insulin secretion in vivo. RESULTS: Certain combinations of the I27L and A98V polymorphisms in the HNF-1alpha gene showed decreased transcriptional activity on the target promoters glucose transporter 2 (now known as solute carrier family 2 [facilitated glucose transporter], member 2) and albumin in both HeLa and INS-1 cells. In vivo, these polymorphisms were associated with a modest but significant impairment in insulin secretion in response to oral glucose. Insulin secretion deteriorated over time in individuals carrying the V allele of the A98V polymorphism (n = 2,293; p = 0.003). In a new case-control (n = 1,511 and n = 2,225 respectively) data set, the I27L polymorphism was associated with increased risk of type 2 diabetes, odds ratio (OR) = 1.5 (p = 0.002; multiple logistic regression), particularly in elderly (age > 60 years) and overweight (BMI > 25 kg/m(2)) patients (OR = 2.3, p = 0.002). CONCLUSIONS/INTERPRETATION: This study provides in vitro and in vivo evidence that common variants in the MODY3 gene, HNF-1alpha, influence transcriptional activity and insulin secretion in vivo. These variants are associated with a modestly increased risk of late-onset type 2 diabetes in subsets of elderly overweight individuals.
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Authors | J Holmkvist, C Cervin, V Lyssenko, W Winckler, D Anevski, C Cilio, P Almgren, G Berglund, P Nilsson, T Tuomi, C M Lindgren, D Altshuler, L Groop |
Journal | Diabetologia
(Diabetologia)
Vol. 49
Issue 12
Pg. 2882-91
(Dec 2006)
ISSN: 0012-186X [Print] Germany |
PMID | 17033837
(Publication Type: Journal Article, Multicenter Study)
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Chemical References |
- Blood Glucose
- Hepatocyte Nuclear Factor 1-alpha
- Insulin
- Arginine
- Glucose
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Topics |
- Adult
- Amino Acid Substitution
- Arginine
(pharmacology)
- Blood Glucose
(metabolism)
- Body Weight
- Case-Control Studies
- Diabetes Mellitus, Type 2
(epidemiology, genetics)
- Female
- Genetic Variation
- Glucose
(pharmacology)
- Hepatocyte Nuclear Factor 1-alpha
(genetics)
- Humans
- Insulin
(metabolism)
- Insulin Secretion
- Male
- Middle Aged
- Mutagenesis, Site-Directed
- Overweight
- Plasmids
- Polymorphism, Single Nucleotide
- Risk Factors
- Transcription, Genetic
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