Abstract | OBJECTIVE: DESIGN: Controlled, randomized animal study. SETTING: University animal care facility and research laboratory. SUBJECT: INTERVENTIONS: MEASUREMENTS AND RESULTS: Using intravital fluorescence microscopy hepatic microcirculation was assessed at baseline, end of shock phase as well as 1 h and 5 h after resuscitation. Systemic arterial blood pressure and bile flow were continuously monitored. 5-AIQ treatment attenuated shock/ resuscitation-induced increase of intrahepatic leukocyte-endothelial cell interaction with a marked reduction of both sinusoidal leukostasis and venular leukocyte adherence. Moreover, nutritive perfusion was found improved, guaranteeing sufficient oxygen supply to tissue, as indicated by low NADH autofluorescence, which was not different to that in controls. Most notably, excretory liver function reached baseline level over 5 h of reperfusion in 5-AIQ-treated animals. CONCLUSIONS: In the present setting of shock/ resuscitation in male rats the PARP inhibitor 5-AIQ proved to be very effective in ameliorating compromised liver microcirculation and function. Further research has to confirm that PARP inhibition is a suitable tool in the acute treatment of patients suffering from reduced circulating blood volume and thus microcirculatory organ dysfunction.
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Authors | J P Roesner, D A Vagts, T Iber, C Eipel, B Vollmar, G F E Nöldge-Schomburg |
Journal | Intensive care medicine
(Intensive Care Med)
Vol. 32
Issue 10
Pg. 1649-57
(Oct 2006)
ISSN: 0342-4642 [Print] United States |
PMID | 16927075
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 5-aminoisoquinolinone
- Isoquinolines
- Poly(ADP-ribose) Polymerase Inhibitors
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Topics |
- Animals
- Isoquinolines
(pharmacology)
- Liver
(blood supply)
- Male
- Microcirculation
(drug effects)
- Microscopy, Fluorescence
- Poly(ADP-ribose) Polymerase Inhibitors
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(prevention & control)
- Resuscitation
- Shock, Hemorrhagic
(physiopathology)
- Statistics, Nonparametric
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