Expression of pituitary adenylate cyclase-activating polypeptide 1 and 2 receptor mRNA in gallbladder tissue of patients with gallstone or gallbladder polyps.

Abstract

AIM:

To detect the expression of pituitary adenylate cyclase-activating polypeptide receptor 1 (VPCAP1-R)and VPCAP2-R mRNA in gallbladder tissues of patients with gallstone or gallbladder polyps.

METHODS:

The expression of VPCAP1-R and VPCAP2-R mRNA in gallbladder tissues was detected in 25 patients with gallstone,8 patients with gallbladder polyps and 7 donors of liver transplantation by reverse transcription polymerase chain reaction (RT-PCR).

RESULTS:

The VPCAP2-R mRNA expression level in the control group (1.09+/-0.58) was lower than that in the gallbladder polyp group (1.64+/-0.56) and the gallstone group (1.55+/-0.45) (P<0.05) while the VPCAP1-R mRNA expression level in the control group (1.15+/-0.23) was not apparently different from that in the gallbladder polyp group (1.28+/-0.56) and the gallstone group (1.27+/-0.38).

CONCLUSION:

The abnormal expression of VPCAP2-R mRNA in gallbladder tissue may play a role in the formation of gallbladder stone and gallbladder polyps.

• Authors: Zhen-Hai Zhang, Shuo-Dong Wu, Hong Gao, Gang Shi, Jun-Zhe Jin, Jing Kong, Zhong Tian, Yang Su
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 12 Issue 9 Pg. 1468-71 (Mar 07 2006) ISSN: 1007-9327 [Print] United States
• PMID: 16552823 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
• Receptors, Vasoactive Intestinal Polypeptide, Type I

Topics

• Adult
• Female
• Gallbladder (chemistry)
• Gallbladder Diseases (genetics, metabolism)
• Gallstones (chemistry, etiology, genetics)
• Gene Expression Regulation
• Humans
• Male
• Middle Aged
• Polyps (chemistry, etiology, genetics)
• RNA, Messenger (analysis)
• Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I (genetics, physiology)
• Receptors, Vasoactive Intestinal Polypeptide, Type I (genetics, physiology)
• Reverse Transcriptase Polymerase Chain Reaction