Abstract |
Human T-cell lymphotropic virus type-1 (HTLV-1)-associated myelopathy/ tropical spastic paraparesis (HAM/TSP) is chronic inflammatory disease of the spinal cord characterized by perivascular lymphocytic cuffing and parenchymal lymphocytic infiltration. In this study using flow cytometry, we have investigated the T-cell receptor (TCR) Vbeta repertoire of peripheral blood T lymphocytes in 8 HAM/TSP patients, 10 HTLV-1 infected healthy carriers, and 11 uninfected healthy controls to determine if there is a biased usage of TCR Vbeta. We found that TCR Vbeta7.2 was under-utilized and Vbeta12 was over-utilized in CD4+ T cells of HTLV-1 infected individuals compared with healthy uninfected controls, whereas there were no such differences in CD8+ T cells. Comparison of Vbeta repertoire changes before and after interferon-alpha (IFN-alpha) treatment for HAM/TSP revealed that one out of five patients showed dramatic decrease of specific Vbeta in CD8+ T cells. Our results suggest that dominant Vbeta subpopulations in CD4+ T cells evolved associated with chronic HTLV-1 infection, and IFN-alpha treatment for HAM/TSP does not induce a specific pattern of TCR Vbeta changes.
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Authors | Mineki Saito, Hirohisa Nose, Koichiro Usuku, Amir H Sabouri, Toshio Matsuzaki, Shuji Izumo, Kimiyoshi Arimura, Mitsuhiro Osame |
Journal | Journal of the neurological sciences
(J Neurol Sci)
Vol. 246
Issue 1-2
Pg. 37-43
(Jul 15 2006)
ISSN: 0022-510X [Print] Netherlands |
PMID | 16545396
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antiviral Agents
- Interferon-alpha
- Receptors, Antigen, T-Cell, alpha-beta
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Topics |
- Antiviral Agents
(therapeutic use)
- CD4-Positive T-Lymphocytes
(metabolism)
- CD8-Positive T-Lymphocytes
(metabolism)
- Flow Cytometry
- HTLV-I Infections
(drug therapy, metabolism)
- Humans
- Interferon-alpha
(therapeutic use)
- Neurologic Examination
- Paraparesis, Tropical Spastic
(drug therapy, metabolism)
- Phenotype
- Receptors, Antigen, T-Cell, alpha-beta
(genetics, metabolism)
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