Abstract | OBJECTIVES: The primary aim of this study was to investigate the expression of apoptotic and cell cycle regulators p53, p21, p27, bax, and bcl-2 in uterine leiomyosarcoma in order to identify molecular pathways that possibly could be important in the development of leiomyosarcoma. A secondary aim was to examine if the apoptotic and cell cycle regulatory protein expression profile of uterine leiomyosarcoma is potentially useful for clinical prognostic purposes. METHODS: A tissue microarray representing 36 uterine leiomyosarcomas and 19 uterine leiomyomas was created with 3 representative cores from each tumor. Immunohistochemical staining was performed for bcl-2, bax, p21, p27, and p53 using standard techniques. Staining was scored 0-12 for each marker, 0-3 being negative and 4-12 positive. Outcome analyses were performed only for leiomyosarcomas. First recurrence was determined from the time of initial diagnosis. Survival was determined from the time of initial diagnosis to last follow-up. RESULTS: Associations were found between disease type ( leiomyosarcoma vs. leiomyoma) and the positivity status of p21 (43% vs. 0%, P < 0.001), p53 (54% vs. 0%, P < 0.001), and bax (34% vs. 94%, P < 0.001). bcl-2-positive leiomyosarcoma was associated with a longer time to recurrence (P = 0.02) in a univariate analysis. In a multivariate analysis, tumor stage was the only independent significant prognostic factor (P = 0.002). CONCLUSION:
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Authors | Aliza L Leiser, Sibyl E Anderson, Daisuke Nonaka, Shaokun Chuai, Adam B Olshen, Dennis S Chi, Robert A Soslow |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 101
Issue 1
Pg. 86-91
(Apr 2006)
ISSN: 0090-8258 [Print] United States |
PMID | 16289259
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Cell Cycle Proteins
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Topics |
- Adult
- Aged
- Apoptosis
(physiology)
- Biomarkers, Tumor
(biosynthesis)
- Cell Cycle
(physiology)
- Cell Cycle Proteins
(biosynthesis)
- Female
- Humans
- Immunohistochemistry
- Leiomyosarcoma
(metabolism, pathology)
- Middle Aged
- Neoplasm Staging
- Prognosis
- Survival Rate
- Uterine Neoplasms
(metabolism, pathology)
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