[Role of estrogen receptor-related receptors alpha, beta and gamma in ovarian cancer cells].

Abstract

OBJECTIVE:

To investigate the role of human estrogen receptor-related receptors (ERRs) in ovarian cancer.

METHODS:

A green fluorescent protein (GFP) reporter plasmid of ERRalpha was constructed and transfected into ovarian cancer cell lines SKOV3 and OVCAR3. RT-PCR was used to analyze the expression of ERRalpha, ERRbeta, and ERRgamma mRNA in 33 primary ovarian cancer samples and 12 normal ovarian epithelial cells. Progression-free survival time and overall survival time of patients with different expression of ERRs were analyzed by Kaplan-Meier method.

RESULTS:

GFP-ERRalpha fusion protein showed ERRalpha expressed in the ovarian cancer cell nucleus. Expressions of the ERRalpha and ERRgamma were increased in the ovarian cancers (58% and 48%) compared with the normal ovarian tissues (P < 0.05); ERRbeta was weakly expressed in the ovarian cancer (9%) as well as in the normal tissues (0). Expression of ERRalpha was positively correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage (r = 0.639, P = 0.017) and cell grading (r = 0.520, P = 0.022). Survival analysis showed that ERRalpha positive group had a poorer overall survival (19.0 months vs. 31.5 months, P < 0.05) and ERRgamma positive group had a longer progression-free survival (18.0 months vs. 13.5 months, P < 0.05).

CONCLUSIONS:

ERRalpha chiefly expresses in the cell nucleus. ERRalpha and ERRgamma may play important roles in ovarian cancer.

• Authors: Peng-ming Sun, Li-hui Wei, Jalid Sehouli, Carsten Denkert, Dan Zhao, Min Gao, Xiu-li Sun, Werner Litchtenegger
• Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi) Vol. 40 Issue 8 Pg. 544-8 (Aug 2005) ISSN: 0529-567X [Print] China
• PMID: 16202294 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ESR1 protein, human
• ESRRG protein, human
• Estrogen Receptor alpha
• Receptors, Estrogen
• estrogen receptor-related receptor beta
• Green Fluorescent Proteins

Topics

• Estrogen Receptor alpha (metabolism)
• Female
• Green Fluorescent Proteins (metabolism)
• Humans
• Ovarian Neoplasms (diagnosis, metabolism)
• Prognosis
• Receptors, Estrogen (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• ERRalpha Estrogen-Related Receptor