Inhibitory effects of costunolide on the telomerase activity in human breast carcinoma cells.

Abstract

Costunolide, a natural sesquiterpene compound, has been known having cytotoxic and chemopreventive effects on various human cancer cells. In the present study, we examined the effects of costunolide on telomerase activity and on the components of telomerase in MCF-7 (wild-type p53) and MDA-MB-231 (mutant p53) cells. We found that costunolide inhibited the growth and telomerase activity of MCF-7 and MDA-MB-231 cells in a concentration- and time-dependent manner. The expression of hTERT mRNA was also inhibited but hTR mRNA was not. In addition, the bindings of transcription factors in hTERT promoters were significantly decreased in both cells by the treatment of costunolide. These results suggest that costunolide inhibited the growth of both MCF-7 and MDA-MB-231 cells and this effect was mediated at least in part by a significant reduction in telomerase activity.

• Authors: Sang-Ho Choi, Eunok Im, Hyun Kyung Kang, Ji-Hyeon Lee, Hi-Suk Kwak, Young-Tae Bae, Hee-Juhn Park, Nam Deuk Kim
• Journal: Cancer letters (Cancer Lett) Vol. 227 Issue 2 Pg. 153-62 (Sep 28 2005) ISSN: 0304-3835 [Print] Ireland
• PMID: 16112418 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• DNA, Neoplasm
• DNA-Binding Proteins
• Proto-Oncogene Proteins c-myb
• RNA, Messenger
• RNA, Neoplasm
• Sesquiterpenes
• Sp1 Transcription Factor
• Transcription Factors
• Tumor Suppressor Protein p53
• telomerase RNA
• Sp3 Transcription Factor
• costunolide
• RNA
• Nitric Oxide Synthase
• Telomerase

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Breast Neoplasms (enzymology, pathology)
• DNA, Neoplasm (genetics, metabolism)
• DNA-Binding Proteins (drug effects, metabolism)
• Female
• Humans
• Nitric Oxide Synthase (antagonists & inhibitors)
• Promoter Regions, Genetic (genetics)
• Proto-Oncogene Proteins c-myb (metabolism)
• RNA (metabolism)
• RNA, Messenger (metabolism)
• RNA, Neoplasm (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Sesquiterpenes (pharmacology)
• Sp1 Transcription Factor (metabolism)
• Sp3 Transcription Factor
• Telomerase (drug effects, metabolism)
• Transcription Factors (metabolism)
• Tumor Cells, Cultured
• Tumor Suppressor Protein p53 (genetics)