Cell line OCI/AML3 bears exon-12 NPM gene mutation-A and cytoplasmic expression of nucleophosmin.

Abstract

We recently identified a new acute myeloid leukemia (AML) subtype characterized by mutations at exon-12 of the nucleophosmin (NPM) gene and aberrant cytoplasmic expression of NPM protein (NPMc+). NPMc+ AML accounts for about 35% of adult AML and it is associated with normal karyotype, wide morphological spectrum, CD34-negativity, high frequency of FLT3-ITD mutations and good response to induction therapy. In an attempt to identify a human cell line to serve as a model for the in vitro study of NPMc+ AML, we screened 79 myeloid cell lines for mutations at exon-12 of NPM. One of these cell lines, OCI/AML3, showed a TCTG duplication at exon-12 of NPM. This mutation corresponds to the type A, the NPM mutation most frequently observed in primary NPMc+ AML. OCI/AML3 cells also displayed typical phenotypic features of NPMc+ AML, that is, expression of macrophage markers and lack of CD34, and the immunocytochemical hallmark of this leukemia subtype, that is, the aberrant cytoplasmic expression of NPM. The OCI/AML3 cell line easily engrafts in NOD/SCID mice and maintains in the animals the typical features of NPMc+ AML, such as the NPM cytoplasmic expression. For all these reasons, the OCI/AML3 cell line represents a remarkable tool for biomolecular studies of NPMc+ AML.

• Authors: H Quentmeier, M P Martelli, W G Dirks, N Bolli, A Liso, R A F Macleod, I Nicoletti, R Mannucci, A Pucciarini, B Bigerna, M F Martelli, C Mecucci, H G Drexler, B Falini
• Journal: Leukemia (Leukemia) Vol. 19 Issue 10 Pg. 1760-7 (Oct 2005) ISSN: 0887-6924 [Print] England
• PMID: 16079892 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD34
• Biomarkers
• NPM1 protein, human
• Nuclear Proteins
• Nucleophosmin

Topics

• Animals
• Antigens, CD34 (metabolism)
• Biomarkers (metabolism)
• Cytoplasm (metabolism)
• DNA Mutational Analysis
• Exons (genetics)
• Gene Expression Regulation, Leukemic
• Humans
• Karyotyping
• Leukemia, Promyelocytic, Acute (genetics, metabolism)
• Macrophages (metabolism)
• Male
• Mice
• Mice, Inbred NOD
• Mice, SCID
• Middle Aged
• Mutation (genetics)
• Nuclear Proteins (genetics, metabolism)
• Nucleophosmin