Abstract |
It is not known whether there is an impairment in vas deferens motility after unilateral testicular torsion/detorsion. Therefore, we aimed to determine whether the electrical field stimulation (EFS)-evoked biphasic contractions are altered in ipsilateral and contralateral rat vasa deferentia obtained from animals exposed to the unilateral testicular torsion/detorsion procedure. We also evaluated the effects of melatonin (MLT), which is a strong antioxidant, on these contractile responses. Rats were subjected to torsion of the left testis for 2 h and then detorsion was performed. Contractility studies were carried out 2 h or 24 h after detorsion. Vas deferens strips were prepared from both the ipsilateral and the contralateral site 2 h or 24 h after the detorsion procedure to record EFS-evoked biphasic twitch responses. The same experimental protocol was repeated for the MLT-treated rats. Both phases of EFS-evoked contractions were decreased after torsion/detorsion in the ipsilateral vas deferens. MLT treatment increased torsion/detorsion-induced reduction of both phases of contractions after 2 h and 24 h. In the contralateral vas deferens, the first phase of EFS-evoked contractions was not changed, while the second phase of contractions was diminished 2 h and 24 h after detorsion. Although MLT decreased the second phase of contractions 2 h and 24 h after detorsion, it reduced the first phase of contractions only 2 h after detorsion. These results suggest that MLT produces an inhibition on EFS-evoked biphasic twitch responses in the ipsilateral and contralateral rat vasa deferentia following unilateral testicular torsion/detorsion in the rat.
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Authors | Süreyya Barun, Gülşen Ekingen, Ismail Mert Vural, Zafer Türkyilmaz, Can Başaklar, Nuri Kale, Zeynep Sevim Ercan, Yusuf Sarioğlu |
Journal | Naunyn-Schmiedeberg's archives of pharmacology
(Naunyn Schmiedebergs Arch Pharmacol)
Vol. 371
Issue 5
Pg. 351-8
(May 2005)
ISSN: 0028-1298 [Print] Germany |
PMID | 16003545
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Electric Stimulation
- Ischemia
- Male
- Melatonin
(pharmacology)
- Muscle Contraction
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Testis
(blood supply)
- Vas Deferens
(drug effects)
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