Abstract | OBJECTIVE: MATERIALS AND METHODS:
VEGF secretion in cultured HPMC, established human OVCA cell lines, and inflammatory or OVCA-associated ascites was determined by enzyme linked immunosorbent assay. RESULTS: HPMC constitutively produced VEGF at median levels of 43 +/- 7 pg/10(5) cells. Treatment of HPMC with 1 ng/ml IL-1beta (567 +/- 213 pg/10(5) cells) or TNF-alpha (89 +/- 1 pg/10(5) cells) resulted in a 13-fold (P < 0.01) or 2-fold (P < 0.05) elevation of the VEGF secretion. In OVCA, the constitutive VEGF expression was 8-fold higher than VEGF levels in HPMC (364 +/- 185 pg/10(5) cells; P < 0.001). VEGF secretion in OVCA cells was also increased by IL-1beta (514 +/- 105 pg/10(5) cells; P < 0.01) or TNF-alpha (458 +/- 168 pg/10(5) cells; P < 0.01) reaching similar levels as in IL-1beta-activated HPMC. Median VEGF levels in malignant ascites (2761 +/- 1549 pg/ml) were 11-fold higher compared with levels in inflammatory fluids (244 +/- 170 pg/ml; P < 0.01). VEGF levels in both inflammatory- and OVCA-associated fluids correlated with ascitic IL-1beta levels (P < 0.05). CONCLUSION: We identified ovarian cancer cells and/or IL-1beta-activated peritoneal mesothelial cells as important sources of ascitic VEGF. The present data indicate that IL-1beta-triggered VEGF production by neoplastic and normal cells is a common pathomechanism for ascites formation in both inflammatory and malignant conditions.
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Authors | Sylvia Stadlmann, Albert Amberger, Juergen Pollheimer, Guenther Gastl, Felix Albert Offner, Raimund Margreiter, Alain Gustave Zeimet |
Journal | Gynecologic oncology
(Gynecol Oncol)
Vol. 97
Issue 3
Pg. 784-9
(Jun 2005)
ISSN: 0090-8258 [Print] United States |
PMID | 15943987
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-1
- Recombinant Proteins
- Vascular Endothelial Growth Factor A
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Topics |
- Ascitic Fluid
(metabolism, pathology)
- Cell Line, Tumor
- Epithelial Cells
(metabolism)
- Female
- Humans
- Interleukin-1
(metabolism, pharmacology)
- Ovarian Neoplasms
(metabolism, pathology)
- Peritoneal Cavity
(cytology)
- Peritonitis
(metabolism, pathology)
- Recombinant Proteins
(pharmacology)
- Secretory Rate
(drug effects)
- Vascular Endothelial Growth Factor A
(biosynthesis, metabolism)
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