CC chemokine receptor 4 ligand production by bronchoalveolar lavage fluid cells in cigarette-smoke-associated acute eosinophilic pneumonia.

Abstract

We examined the production of macrophage-derived chemokine (MDC/CCL22) and thymus- and activation-regulated chemokine (TARC/CCL17) by bronchoalveolar lavage fluid (BALF) cells in cigarette-smoke-associated acute eosinophilic pneumonia (CS-AEP). The CC Chemokine Receptor 4 (CCR4) ligand levels in BALF from patients with CS-AEP were considerably higher than those in healthy volunteers and correlated well with Th2 cytokine levels. Interleukin-4 enhanced CCR4 ligand production. MDC expression was observed in CD68-positive cells from patients with CS-AEP and in healthy control smokers. In contrast, TARC expression in CD68- or CD1a-positive cells was detected only in CS-AEP. An in vivo cigarette smoke challenge test induced increases in CCR4 ligands in the BALF and in the cultured supernatant of BALF adherent cells. These results suggest that alveolar macrophages and dendritic cells contribute to the pathogenesis of CS-AEP by generating CCR4 ligands, probably in response to cigarette smoke.

• Authors: Shin-ichi Nureki, Eishi Miyazaki, Masaru Ando, Toshihide Kumamoto, Tomiyasu Tsuda
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 116 Issue 1 Pg. 83-93 (Jul 2005) ISSN: 1521-6616 [Print] United States
• PMID: 15925835 (Publication Type: Journal Article)

Chemical References

• CCL17 protein, human
• CCL22 protein, human
• CCR4 protein, human
• Chemokine CCL17
• Chemokine CCL22
• Chemokines, CC
• Ligands
• Receptors, CCR4
• Receptors, Chemokine
• Interleukin-4
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Adolescent
• Adult
• Bronchoalveolar Lavage Fluid (immunology)
• Chemokine CCL17
• Chemokine CCL22
• Chemokines, CC (metabolism)
• Granulocyte-Macrophage Colony-Stimulating Factor (metabolism)
• Humans
• Immunohistochemistry
• Interleukin-4 (metabolism)
• Ligands
• Pulmonary Eosinophilia (etiology, metabolism)
• Receptors, CCR4
• Receptors, Chemokine (metabolism)
• Smoking (adverse effects, metabolism)
• T-Lymphocyte Subsets (metabolism)
• Th2 Cells (metabolism)