Abstract |
The activity of inosine-5'-monophosphate dehydrogenase (IMPDH) inhibitors, mizoribine and ribavirin, against severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) was determined by plaque reduction and yield reduction assays. Mizoribine and ribavirin selectively inhibited replication of SARS-CoV. The 50% inhibitory concentration (IC50) of mizoribine for SARS-CoV Frankfurt-1 and SARS-CoV HKU39849, as determined by plaque reduction was 3.5 microg/ml and 16 microg/ml, respectively, and the IC50 of ribavirin for SARS-CoV Frankfurt-1 and SARS-CoV HKU39849 was 20 microg/ml and 80 microg/ml, while the 50% cytotoxic concentration of mizoribine and ribavirin for Vero E6 cells exceeded 200 microg/ml. In a yield reduction assay, mizoribine (10 microg/ml) and ribavirin (40 microg/ml) inhibited the replication of SARS-CoV and reduced the infectious SARS-CoV titers to one-tenth or less. Mizoribine inhibited replication of SARS-CoV more strongly than ribavirin. However, neither drug could completely inhibit replication of SARS-CoV even at concentrations up to 100 microg/ml.
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Authors | Masayuki Saijo, Shigeru Morikawa, Shuetsu Fukushi, Tetsuya Mizutani, Hideki Hasegawa, Noriyo Nagata, Naoko Iwata, Ichiro Kurane |
Journal | Antiviral research
(Antiviral Res)
Vol. 66
Issue 2-3
Pg. 159-63
(Jun 2005)
ISSN: 0166-3542 [Print] Netherlands |
PMID | 15911031
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antiviral Agents
- Ribonucleosides
- Ribavirin
- mizoribine
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Topics |
- Animals
- Antiviral Agents
(chemistry, pharmacology)
- Chlorocebus aethiops
- Microbial Sensitivity Tests
- Ribavirin
(chemistry, pharmacology)
- Ribonucleosides
(chemistry, pharmacology)
- Severe acute respiratory syndrome-related coronavirus
(drug effects, enzymology, genetics, physiology)
- Vero Cells
- Virus Replication
(drug effects)
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