Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS.

Abstract

The recombinant nucleocapsid protein (rNP) of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) was expressed in a baculovirus system. The purified SARS-CoV rNP was used as an antigen for detection of SARS-CoV antibodies in IgG enzyme-linked immunosorbent assay (ELISA). The ELISA was evaluated in comparison with neutralizing antibody assay and the authentic SARS-CoV antigen-based IgG ELISA. Two-hundred and seventy-six serum samples were collected from health care workers in a hospital in which a nosocomial SARS outbreak took place and used for evaluation. The SARS-CoV rNP-based IgG ELISA has 92% of sensitivity and specificity compared with the neutralizing antibody assay and 94% sensitivity and specificity compared with the authentic SARS-CoV antigen-based IgG ELISA. The results suggest that the newly developed SARS-CoV rNP-based IgG ELISA is a valuable tool for the diagnosis and seroepidemiological study of SARS. The SARS-CoV rNP-based IgG ELISA has an advantage over the conventional IgG ELISA in that the antigen can be prepared by laboratory workers without the risk of infection.

• Authors: Masayuki Saijo, Toshio Ogino, Fumihiro Taguchi, Shuetsu Fukushi, Tetsuya Mizutani, Tsugunori Notomi, Hidetoshi Kanda, Harumi Minekawa, Shutoku Matsuyama, Hoang Thuy Long, Nguyen Thi Hong Hanh, Ichiro Kurane, Masato Tashiro, Shigeru Morikawa
• Journal: Journal of virological methods (J Virol Methods) Vol. 125 Issue 2 Pg. 181-6 (May 2005) ISSN: 0166-0934 [Print] Netherlands
• PMID: 15794988 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Coronavirus Nucleocapsid Proteins
• Immunoglobulin G
• Nucleocapsid Proteins
• Recombinant Proteins

Topics

• Animals
• Antibodies, Viral
• Chlorocebus aethiops
• Coronavirus Nucleocapsid Proteins
• Enzyme-Linked Immunosorbent Assay (methods)
• Immunoglobulin G (blood)
• Nucleocapsid Proteins (immunology)
• Recombinant Proteins (immunology)
• Severe acute respiratory syndrome-related coronavirus (immunology, isolation & purification)
• Severe Acute Respiratory Syndrome (diagnosis, immunology)
• Vero Cells