Abstract | BACKGROUND: METHODS AND RESULTS: This study was aimed at evaluating the efficacy and safety of an adenoviral vector (Ad2/ betaARKct) encoding the carboxyl terminus of beta-adrenergic receptor kinase ( betaARKct) in a pig model of arteriovenous PTFE graft failure. Transduction of the external jugular vein with Ad2/ betaARKct (5E9, 5E10, or 5E11 particles per vein) did not result in systemic toxicity, as measured by clinical and pathological assessments. Ad2/ betaARKct significantly reduced neointimal hyperplasia in the graft/vein anastomosis. It also improved the graft patency rate and angiographic score, as measured histologically and angiographically, compared with vehicle or empty viral vector controls. CONCLUSIONS: Our results suggest that local administration of adenoviral vectors encoding betaARKct into the jugular vein represents a viable strategy to treat AV graft hemodialysis vascular access failure.
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Authors | Zhengyu Luo, Geoffrey Y Akita, Taro Date, Christopher Treleaven, Karen A Vincent, Denise Woodcock, Seng H Cheng, Richard J Gregory, Canwen Jiang |
Journal | Circulation
(Circulation)
Vol. 111
Issue 13
Pg. 1679-84
(Apr 05 2005)
ISSN: 1524-4539 [Electronic] United States |
PMID | 15781730
(Publication Type: Journal Article)
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Chemical References |
- Receptors, Adrenergic, beta
- Polytetrafluoroethylene
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Topics |
- Adenoviridae
(genetics)
- Animals
- Arteriovenous Shunt, Surgical
(adverse effects)
- Catheters, Indwelling
(adverse effects)
- Equipment Failure
- Gene Expression Regulation
- Graft Occlusion, Vascular
(therapy)
- Hyperplasia
(therapy)
- Polytetrafluoroethylene
(therapeutic use)
- Receptors, Adrenergic, beta
(administration & dosage)
- Renal Dialysis
- Swine
- Transduction, Genetic
- Tunica Intima
(pathology)
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