Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS: Treatment of 4T1 cells with EGCG resulted in inhibition of cell proliferation, induction of apoptosis in dose- and time-dependent manner. The increase in apoptosis was accompanied with decrease in the protein expression of Bcl-2 concomitantly increase in Bax, cytochrome c release, Apaf-1, and cleavage of caspase 3 and PARP proteins. Treatment of EGCG-rich GTP in drinking water to 4T1 cells bearing BALB/c mice resulted in reduction of tumor growth accompanied with increase in Bax/Bcl-2 ratio, reduction in proliferating cell nuclear antigen and activation of caspase 3 in tumors. Metastasis of tumor cells to lungs was inhibited and survival period of animals was increased after green tea treatment. CONCLUSION: This study suggests that GTP have the ability to prevent the development of breast cancer and its metastasis; however, further in vivo studies are required to identify the molecular targets.
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Authors | Manjeshwar S Baliga, Sreelatha Meleth, Santosh K Katiyar |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 11
Issue 5
Pg. 1918-27
(Mar 01 2005)
ISSN: 1078-0432 [Print] United States |
PMID | 15756018
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Flavonoids
- Phenols
- Polyphenols
- Proliferating Cell Nuclear Antigen
- Tea
- Casp3 protein, mouse
- Caspase 3
- Caspases
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Topics |
- Administration, Oral
- Animals
- Apoptosis
- Breast Neoplasms
- Carcinoma
(pathology)
- Caspase 3
- Caspases
(pharmacology)
- Cell Proliferation
- Female
- Flavonoids
(pharmacology)
- Gene Expression Profiling
- Mammary Neoplasms, Animal
(pathology)
- Mice
- Mice, Inbred BALB C
- Neoplasm Metastasis
- Phenols
(pharmacology)
- Polyphenols
- Proliferating Cell Nuclear Antigen
(biosynthesis)
- Tea
(chemistry)
- Tumor Cells, Cultured
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