Dimethylsulfoxide induces upregulation of tumor suppressor protein PTEN through nuclear factor-kappaB activation in HL-60 cells.

Abstract

Dimethylsulfoxide (DMSO) has been known to differentiate HL60 cells into neutrophil like cells. Here, we provide an evidence for the involvement of tumor suppressor PTEN, an antagonist of phosphatidylinositol 3-kinase (PI3K) in the DMSO-induced differentiation of HL60 cells. DMSO upregulated PTEN with unaffecting the expression of PI3K. The upregulation of PTEN by DMSO lead to the decrease of Akt phosphorylation, a downstream of PI3K. The DMSO-induced upregulation of PTEN might be mediated by NF-kappaB activation, which was evidenced by the blockage of DMSO-induced PTEN upregulation with an NF-kappaB inhibitor, pyrrolidine dithiocarbamate (PDTC).

• Authors: Young-Rae Lee, Hyun-Jaung Shim, Hong-Nu Yu, Eun-Kyung Song, Jinny Park, Kang-Beom Kwon, Jin-Woo Park, Hye-Won Rho, Byung-Hyun Park, Myung-Kwan Han, Jong-Suk Kim
• Journal: Leukemia research (Leuk Res) Vol. 29 Issue 4 Pg. 401-5 (Apr 2005) ISSN: 0145-2126 [Print] England
• PMID: 15725474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• NF-kappa B
• Phosphoinositide-3 Kinase Inhibitors
• Tumor Suppressor Proteins
• Phosphoric Monoester Hydrolases
• PTEN Phosphohydrolase
• PTEN protein, human
• Dimethyl Sulfoxide

Topics

• Base Sequence
• DNA Primers
• Dimethyl Sulfoxide (pharmacology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Genes, Tumor Suppressor (drug effects)
• HL-60 Cells
• Humans
• Kinetics
• NF-kappa B (metabolism)
• PTEN Phosphohydrolase
• Phosphoinositide-3 Kinase Inhibitors
• Phosphoric Monoester Hydrolases (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Suppressor Proteins (genetics)