Inhibitory effects of OK-432 (Picibanil) on cellular proliferation and adhesive capacity of breast carcinoma cells.

Abstract

We investigated the potent inhibitory effects of OK-432 (Picibanil) on both cellular adhesion and cell proliferation of estrogen-dependent (MCF-7) or estrogen-independent (MDA-MB-231) breast carcinoma cells. Cellular proliferation of both MCF-7 and MDA-MB-231 cells was markedly inhibited in a dose-dependent manner, when the carcinoma cells were exposed to OK-432. Cell attachment assay demonstrated that incubation with OK-432 for 24 h reduced integrin-mediated cellular adhesion of both cell types. However, fluorescence activated cell sorter (FACS) analysis revealed that incubation with OK-432 for 24 h did not decrease the cell surface expressions of any integrins. These results suggest that the binding avidity of integrins is reduced by OK-432 without alteration of the integrin expression. We conclude that OK-432 inhibits integrin-mediated cellular adhesion as well as cell proliferation of breast carcinoma cells regardless of estrogen-dependence, and that these actions of OK-432 contribute to prevention or inhibition of breast carcinoma invasion and metastasis.

• Authors: Yoshio Horii, Yuichi Iino, Michio Maemura, Jun Horiguchi, Yasuo Morishita
• Journal: Oncology reports (Oncol Rep) Vol. 13 Issue 2 Pg. 289-94 (Feb 2005) ISSN: 1021-335X [Print] Greece
• PMID: 15643513 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Estrogens
• Picibanil

Topics

• Antineoplastic Agents (pharmacology)
• Breast Neoplasms (drug therapy)
• Cell Adhesion (drug effects)
• Cell Proliferation (drug effects)
• Estrogens (pharmacology)
• Female
• Humans
• Neoplasms, Hormone-Dependent (drug therapy)
• Picibanil (pharmacology)
• Tumor Cells, Cultured