Abstract | PURPOSE: METHODS: Diabetes was induced in rats by intraperitoneal injection of 60 mg/kg of streptozotocin. Rats were divided into four groups: controls; untreated diabetes; diabetes treated with FP15 (10 mg/kg oral gavage twice daily) and diabetes treated with PJ34 (10 mg/kg oral gavage twice daily). All experiments were performed 4 weeks after initiation of treatment. Leukocyte entrapment in the retinal microcirculation was quantitatively evaluated in vivo with acridine orange digital fluorography. RESULTS: The density of leukocytes trapped in the retinal microcirculation 30 minutes after dye injection was significantly greater in untreated diabetes (32.1 +/- 4.7 cells/mm2) than in controls (11.3 +/- 4.5 cells/mm2) (p < 0.05). Compared with untreated diabetes, the density of trapped leukocytes significantly decreased in diabetes treated with FP15 (14.5 +/- 5.1 cells/mm2) (p < 0.0001) and diabetes treated with PJ34 (24.1 +/- 4.2 cells/mm2) (p < 0.05). CONCLUSIONS: Treatment with FP15 and PJ34 decreased enhanced leukocyte entrapment in the retinal microcirculation during the early diabetic period. The current study suggests a role for peroxynitrite production and for PARP activation in the pathogenesis of retinal microvascular leukostasis in early diabetes.
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Authors | R Sugawara, T Hikichi, N Kitaya, F Mori, T Nagaoka, A Yoshida, C Szabo |
Journal | Current eye research
(Curr Eye Res)
Vol. 29
Issue 1
Pg. 11-6
(Jul 2004)
ISSN: 0271-3683 [Print] England |
PMID | 15370362
(Publication Type: Journal Article)
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Chemical References |
- Coloring Agents
- FeCl tetrakis-2-(triethyleneglycolmonomethylether)pyridylporphyrin
- Metalloporphyrins
- N-(oxo-5,6-dihydrophenanthridin-2-yl)-N,N-dimethylacetamide hydrochloride
- Phenanthrenes
- Poly(ADP-ribose) Polymerase Inhibitors
- Peroxynitrous Acid
- Acridine Orange
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Topics |
- Acridine Orange
- Administration, Oral
- Animals
- Cell Adhesion
(drug effects)
- Coloring Agents
- Diabetes Mellitus, Experimental
(drug therapy, metabolism)
- Diabetic Retinopathy
(metabolism, prevention & control)
- Fluorophotometry
- Leukocytes
(metabolism)
- Leukostasis
(metabolism)
- Male
- Metalloporphyrins
(pharmacology)
- Microcirculation
- Peroxynitrous Acid
(metabolism)
- Phenanthrenes
(pharmacology)
- Poly(ADP-ribose) Polymerase Inhibitors
- Rats
- Rats, Inbred BN
- Retinal Vessels
(metabolism)
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