Abstract |
Junctional adhesion molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. In the present work, we found that DCs also express JAM-A. To evaluate the biological relevance of this observation, Jam-A(-/-) mice were generated and the functional behavior of DCs in vitro and in vivo was studied. In vitro, Jam-A(-/-) DCs showed a selective increase in random motility and in the capacity to transmigrate across lymphatic endothelial cells. In vivo, Jam-A(-/-) mice showed enhanced DC migration to lymph nodes, which was not observed in mice with endothelium-restricted deficiency of the protein. Furthermore, increased DC migration to lymph nodes was associated with enhanced contact hypersensitivity (CHS). Adoptive transfer experiments showed that JAM-A-deficient DCs elicited increased CHS in Jam-A(+/+) mice, further supporting the concept of a DC-specific effect. Thus, we identified here a novel, non-redundant role of JAM-A in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
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Authors | Maria Rosaria Cera, Annalisa Del Prete, Annunciata Vecchi, Monica Corada, Ines Martin-Padura, Toshiyuki Motoike, Paolo Tonetti, Gianfranco Bazzoni, William Vermi, Francesca Gentili, Sergio Bernasconi, Thomas N Sato, Alberto Mantovani, Elisabetta Dejana |
Journal | The Journal of clinical investigation
(J Clin Invest)
Vol. 114
Issue 5
Pg. 729-38
(Sep 2004)
ISSN: 0021-9738 [Print] United States |
PMID | 15343392
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Adhesion Molecules
- Junctional Adhesion Molecules
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Topics |
- Animals
- Cell Adhesion Molecules
(deficiency, genetics, immunology)
- Cell Movement
(genetics, immunology)
- Dendritic Cells
(immunology)
- Dermatitis, Contact
(genetics, immunology)
- Endothelial Cells
- Fluorescent Antibody Technique
- Junctional Adhesion Molecules
- Lymph Nodes
(immunology)
- Mice
- Mice, Knockout
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