Abstract | BACKGROUND & AIMS: METHODS: Immunohistochemical evaluation of 413 gastric cancers was carried out by using a monoclonal antibody to the epidermal growth factor receptor. The intensity of reactivity was scored by using a 4-tier system (negative, 1+, 2+, and 3+). All positive staining (>1+) tumors overexpressing the protein were then analyzed for gene amplification by fluorescence in situ hybridization by using a gene-specific probe. RESULTS: High levels of overexpression (2+ or 3+ staining) were found in 9 of 413 (2.2%) patients, whereas low levels of overexpression (1+) were found in 34 (8.2%) of the study cohort. Fluorescence in situ hybridization analysis showed that more than 10 copies of the gene were recognized in all 5 cancers with 3+ staining and in 2 of the 4 tumors with 2+ staining. CONCLUSIONS:
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Authors | Takuo Takehana, Kazuyoshi Kunitomo, Shioto Suzuki, Koji Kono, Hideki Fujii, Yoshiro Matsumoto, Akishi Ooi |
Journal | Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association
(Clin Gastroenterol Hepatol)
Vol. 1
Issue 6
Pg. 438-45
(Nov 2003)
ISSN: 1542-3565 [Print] United States |
PMID | 15017643
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Fluorescent Dyes
- ErbB Receptors
- Eosine Yellowish-(YS)
- Hematoxylin
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Topics |
- Adenocarcinoma
(classification, epidemiology, metabolism)
- Adenocarcinoma, Mucinous
(classification, epidemiology, metabolism)
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(analysis)
- Carcinoma, Papillary
(classification, epidemiology, metabolism)
- Carcinoma, Signet Ring Cell
(classification, epidemiology, metabolism)
- Eosine Yellowish-(YS)
- ErbB Receptors
(biosynthesis)
- Female
- Fluorescent Dyes
- Gene Amplification
- Hematoxylin
- Humans
- Immunohistochemistry
- In Situ Hybridization, Fluorescence
- Japan
- Lymphatic Metastasis
- Male
- Middle Aged
- Observer Variation
- Statistics as Topic
- Stomach Neoplasms
(classification, epidemiology, metabolism)
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