Hippocampal heme oxigenase-1 in a murine cardiac arrest model.

Abstract

Heme oxygenase-1 (HO-1) is a member of the heat shock protein family (HSP-32). It responds to thermal stress in cultured glial cells. To our knowledge. nothing is known about the expression and response of the HO-1 in cerebral ischemia. Therefore, we show here the induction of HO-1 in the brain of mice after global cerebral ischemia. HO-1-like immunoreactivity was detected at 12, 24, and 48 hours after ischemia recirculation. The HO-1-like immunoreactive cells were observed in astrocytes in the hippocampal dentate gyrus and CA1. The peak level of HO-1-like immunoreactivity was found 48 hours after the recirculation. HO-1-like immunoreactivity was observed in GFAP-positive astrocytes by use of a double immunostaining method. These results provide direct evidence for the induction and localization of HO-1 immunoreactivity in vivo in a mouse cerebral ischemia. We suggest that HO-1, produced in astrocytes after ischemia-recirculation, may directly affect neurons to protect from cell death.

• Authors: Y Imaizumi, H Mizushima, K Dohi, H Ohtaki, H Funahashi, S Shioda
• Journal: Acta neurochirurgica. Supplement (Acta Neurochir Suppl) Vol. 86 Pg. 101-3 ( 2003) ISSN: 0065-1419 [Print] Austria
• PMID: 14753415 (Publication Type: Journal Article)

Chemical References

• Membrane Proteins
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1
• Hmox1 protein, mouse

Topics

• Animals
• Brain Ischemia (enzymology, etiology)
• Heart Arrest, Induced
• Heme Oxygenase (Decyclizing) (metabolism)
• Heme Oxygenase-1
• Hippocampus (enzymology)
• Immunohistochemistry
• Male
• Membrane Proteins
• Mice
• Mice, Inbred BALB C