Expression and cellular localization of p8 protein in thyroid neoplasms.

Abstract

OBJECTIVES:

p8 protein has mitogenic activity and is linked to the development of pancreatic carcinoma. However, little is known about the expression and physiological significance of this protein in other human carcinomas.

METHODS:

In this study, we immunohistochemically investigated p8 expression in thyroid neoplasms as well as in the normal thyroid gland.

RESULTS:

p8 was expressed in normal follicular cells, but no normal thyroid was regarded as overexpressing p8. On the other hand, 44.3% of papillary carcinoma overexpressed p8 and the incidence was directly linked to the tumor size (p=0.0340) and lymph node metastasis (p=0.0145). In follicular tumors, the incidence of p8 overexpression did not depend on histological type. In anaplastic (undifferentiated) carcinoma, p8 was overexpressed only in 5.0%, which was significantly lower than in papillary (p=0.0006) and follicular carcinomas (p=0.0049). In normal follicules and follicular tumors, p8 was localized mainly in the nucleus except for two adenomas. On the other hand, p8 localization was more cytoplasmic in papillary carcinoma larger than 1.0 cm (p=0.0186) and with a poorly differentiated lesion (p=0.0313).

CONCLUSIONS:

These results suggest that the overexpression and cytoplasmic localization of p8 protein may reflect disease progression of papillary carcinoma, whereas this protein plays little part in thyroid carcinoma after anaplastic transformation.

• Authors: Yasuhiro Ito, Hiroshi Yoshida, Yoshiharu Motoo, Eiji Miyoshi, Juan Lucio Iovanna, Chisato Tomoda, Takashi Uruno, Yuuki Takamura, Akihiro Miya, Kaoru Kobayashi, Fumio Matsuzuka, Nariaki Matsuura, Kanji Kuma, Akira Miyauchi
• Journal: Cancer letters (Cancer Lett) Vol. 201 Issue 2 Pg. 237-44 (Nov 25 2003) ISSN: 0304-3835 [Print] Ireland
• PMID: 14607339 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Basic Helix-Loop-Helix Transcription Factors
• Biomarkers, Tumor
• DNA-Binding Proteins
• High Mobility Group Proteins
• NUPR1 protein, human
• Neoplasm Proteins

Topics

• Adenocarcinoma, Follicular (metabolism, pathology)
• Adenoma (metabolism, pathology)
• Basic Helix-Loop-Helix Transcription Factors
• Biomarkers, Tumor (metabolism)
• Carcinoma (metabolism, pathology)
• Carcinoma, Papillary (metabolism, pathology)
• Cell Nucleus (metabolism)
• Cytoplasm (metabolism)
• DNA-Binding Proteins (metabolism)
• High Mobility Group Proteins (metabolism)
• Humans
• Immunoenzyme Techniques
• Lymphatic Metastasis (pathology)
• Neoplasm Invasiveness
• Neoplasm Proteins
• Protein Transport
• Thyroid Gland (metabolism, pathology)
• Thyroid Neoplasms (metabolism, pathology)