Identification of epithelial cell adhesion molecule autoantibody in patients with ovarian cancer.

Abstract

The epithelial cell adhesion molecule (Ep-CAM) exhibited an ovarian cancer:normal human ovarian surface epithelium ratio of 444. For validation studies, real-time quantitative PCR analysis and immunohistochemistry were performed in normal and malignant ovarian epithelial cell lines and tissues. To evaluate the potential of the Ep-CAM autoantibody as a tumor marker, we examined the amount of Ep-CAM autoantibody in serum samples obtained from ovarian cancer patients and normal controls by an ELISA. Real-time quantitative PCR analysis revealed significant overexpression of Ep-CAM mRNA in cancer cell lines (P < 0.001) and microdissected cancer tissues (P < 0.05), compared with that in cultured normal human ovarian surface epithelium and microdissected germinal epithelium, respectively. Immunolocalization of the Ep-CAM autoantibody showed that the sera of ovarian cancer patients expressed higher levels of Ep-CAM autoantibody than benign tumor patients and normal controls (P < 0.05). The levels of Ep-CAM autoantibody found were as follows: 0.132 in 52 patients with ovarian cancer, 0.098 in 26 cases with benign gynecologic disease, and 0.090 in 26 normal women. This investigation has shown that the Ep-CAM autoantibody was found to be associated with ovarian cancer and suggested that future research assessing its clinical usefulness would be worthwhile.

• Authors: Jae-Hoon Kim, Dorothee Herlyn, Kwong-kwok Wong, Dong-Choon Park, John O Schorge, Karen H Lu, Steven J Skates, Daniel W Cramer, Ross S Berkowitz, Samuel C Mok
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 9 Issue 13 Pg. 4782-91 (Oct 15 2003) ISSN: 1078-0432 [Print] United States
• PMID: 14581349 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• Autoantibodies
• Cell Adhesion Molecules
• Epithelial Cell Adhesion Molecule
• RNA, Messenger

Topics

• Antigens, Neoplasm (immunology)
• Autoantibodies (blood)
• Cell Adhesion Molecules (immunology)
• Cell Line, Tumor
• Enzyme-Linked Immunosorbent Assay
• Epithelial Cell Adhesion Molecule
• Female
• Humans
• Immunohistochemistry
• Nucleic Acid Hybridization
• Oligonucleotide Array Sequence Analysis
• Ovarian Neoplasms (blood, immunology, pathology)
• Ovary (pathology)
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction